Staphylococcus aureus whole genome sequence-based susceptibility and resistance prediction using a clinically amenable workflow.

Staphylococcus aureus whole genome sequence-based susceptibility and resistance prediction using a clinically amenable workflow.

We used graphical person interface-based automated analytical instruments from Subsequent Gen Diagnostics (Mountain View, CA) and 1928 Diagnostics (Gothenburg, Sweden) to investigate entire genome sequence (WGS) knowledge from 102 distinctive blood tradition isolates of Staphylococcus aureus to foretell antimicrobial susceptibly, with outcomes in comparison with these of phenotypic susceptibility testing. Of 916 isolate/antibiotic mixtures analyzed utilizing the Subsequent Gen Diagnostics software, there have been 9 discrepancies between WGS predictions and phenotypic susceptibility/resistance, together with eight for clindamycin and 1 for minocycline. Of 612 isolate/antibiotic mixtures analyzed utilizing the 1928

Diagnostics software, there have been 13 discrepancies between WGS predictions and phenotypic susceptibility/resistance, together with 9 for clindamycin, Three for trimethoprim-sulfamethoxazole, and 1 for rifampin. Trimethoprim-sulfamethoxazole was not assessed by Subsequent Gen Diagnostics, and minocycline was not assessed by 1928 Diagnostics. There was full concordance between phenotypic susceptibility/resistance and genotypic prediction of susceptibility/resistance utilizing each analytical platforms for oxacillin, vancomycin, and mupirocin, in addition to by the Subsequent Gen Diagnostics analytical software for levofloxacin (the 1928 Diagnostics software didn’t assess levofloxacin). 

An intensive vary of biomaterials, regularly derived from extracellular matrix (ECM) proteins or different pure biopolymers, have been developed for biomedical functions. Their mechanical response, a key requirement for regenerative medication, is commonly stiff however reveals restricted extensibility (e.g. silk), or inversely, is compliant with increased pressure to failure (e.g. elastin). Whereas artificial biocompatible supplies exhibiting a mechanical response between these boundaries are uncommon, a number of organic supplies show sudden mixtures of those properties. These outcomes counsel that, from a efficiency standpoint, with some caveats, computerized bioinformatics instruments could also be acceptable to foretell susceptibility and resistance to a panel of antibiotics for S. aureus.

With a purpose to replicate these efficiency metrics in artificial methods, a central requirement is to first reveal the molecular design of their constituent constructing blocks, which has historically been an especially time-consuming activity. Right here, we spotlight the latest software of Subsequent Gen sequencing applied sciences for the characterization of a number of protein-based pure biopolymers, a method which circumvents this analysis bottleneck. Profitable molecular biomimicry of those mannequin protein methods may thus have the potential to considerably increase the vary of intrinsic materials properties obtainable for biomedical functions.

A genomic perspective on the taxonomy of the subtribe Carcharodina (Lepidoptera: Hesperiidae: Carcharodini).

We obtained entire genome shotgun sequences and phylogenetically analyzed protein-coding areas of consultant skipper butterflies from the genus Carcharodus Hübner, [1819] and its shut kinfolk. Sort species of all obtainable genus-group names had been sequenced. We discover that species attributed to 4 solely Outdated World genperiod (Spialia Swinhoe, 1912, Gomalia Moore, 1879, Carcharodus Hübner, [1819] and Muschampia Tutt, 1906) type a monophyletic group that we name a subtribe Carcharodina Verity, 1940. Within the phylogenetic timber constructed from numerous genomic areas, these species type 7 (not 4) teams that we deal with as genperiod. We discover that Muschampia Tutt, 1906 is just not monophyletic, and the fifth group is shaped by at present monotypic genus Favria Tutt, 1906 new standing (sort species Hesperia cribrellum Eversmann, 1841), which is sister to Gomalia.

The sixth and seventh teams are composed of largely African species presently positioned in Spialia. These teams don’t have names and are described right here as Ernsta Grishin, gen. n. (sort species Pyrgus colotes Druce, 1875) and Agyllia Grishin, gen. n. (sort species Pyrgus agylla Trimen, 1889). Two subgroups are acknowledged in Ernsta: the nominal subgenus and a brand new one: Delaga Grishin, subgen. n. (sort species Pyrgus delagoae Trimen, 1898). Subsequent, we observe that Carcharodus is just not monophyletic, and species previously positioned in subgenperiod Reverdinus Ragusa, 1919 and Lavatheria Verity, 1940 are right here transferred to Muschampia.

Moreover, resulting from variations in male genitalia or DNA sequences, we reinstate Gomalia albofasciata Moore, 1879 and Gomalia jeanneli (Picard, 1949) as species, not subspecies or synonyms of Gomalia elma (Trimen, 1862), and Spialia bifida (Higgins, 1924) as a species, not subspecies of Spialia zebra (Butler, 1888). Sequencing of the kind specimens reveals 2.2-3.2% distinction in COI barcodes, the proof that mixed with wing sample variations suggests a brand new standing of a species for Spialia lugens (Staudinger, 1886) and Spialia carnea (Reverdin, 1927), previously subspecies of Spialia orbifer (Hübner, [1823]).

Staphylococcus aureus whole genome sequence-based susceptibility and resistance prediction using a clinically amenable workflow.

Entire-Genome Uterine Artery Transcriptome Profiling and Various Splicing Evaluation in Rat Being pregnant.

Throughout being pregnant, the uterine artery (UA) undergoes in depth reworking to allow a 20-40 fold enhance in blood stream with related modifications within the expression of a large number of genes. This research used subsequentgen RNA sequencing know-how to establish pathways and genes doubtlessly concerned in arterial diversifications in pregnant rat UA (gestation day 20) in contrast with non-pregnant rat UA (diestrus). A complete of 2245 genes had been differentially expressed, with 1257 up-regulated and 970 down-regulated in pregnant UA. Gene clustering evaluation revealed a singular cluster of suppressed genes implicated in calcium signaling pathway and vascular easy muscle contraction in pregnant UA.

TBE-Buffer 5X for DNA Sequencing

40121072-4 4 L
EUR 192.14

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056
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  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

pOET Sequencing Primers

GWB-200100 2 x 100 ul Ask for price

SequaGel Sequencing System 1L Kit - 1KIT

NAT1136 1KIT
EUR 187.65

SequaGel Sequencing System 2.2L Kit - EACH

NAT1138 EACH
EUR 318.6

sequencing unit 33x45 cm

ESEQ1100-SYS ea
EUR 1842

Sequencing Grade Chymotrypsin

RE013 100ug
EUR 309

sequencing system 20x50 cm

ESEQ1200-SYS ea
EUR 1842

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 174

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 262.8

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1988.4

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 781.2

Sequencing Grade V8 Protease

RE014 50ug
EUR 209

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 375.6

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 343.2

Sequencing Grade Modified Trypsin

RE012 100ug
EUR 229

Sequencing Grade Carboxypeptidase B

RE011 100ug
EUR 99

ACES Sequencing Buffer 10X, pH 7.7

21420000-1 1 L
EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7

21420000-2 4 L
EUR 456.17

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ100UG each
EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ1MG each
EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ400UG each
EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

17068 5 nmoles
EUR 2091

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY100UG each
EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY1MG each
EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY400UG each
EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Genorise® Customized PCR primers for cloning and sequencing

GR108023 2 nmol
EUR 65

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

IBOCHYTLCKSG100UG each
EUR 297
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

IBCTCLOSGPF10UG each
EUR 165
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY250UG each
EUR 784
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY50UG each
EUR 304
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

Cell Cycle & DNA Content Solution

PI/RNASE 200 test
EUR 104.5
Description: PI/RNAse

Mouse GC- rich sequence DNA- binding factor, Tcf9 ELISA KIT

ELI-12525m 96 Tests
EUR 1038

Human GC- rich sequence DNA- binding factor, TCF9 ELISA KIT

ELI-27490h 96 Tests
EUR 988.8

Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT

ELI-37633m 96 Tests
EUR 1038

Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT

ELI-08155h 96 Tests
EUR 988.8

Mouse GC-rich sequence DNA-binding factor 1 (GCFC1) ELISA Kit

abx524989-96tests 96 tests
EUR 687.5

Mouse GC-rich sequence DNA-binding factor 2 (GCFC2) ELISA Kit

abx524991-96tests 96 tests
EUR 687.5

20 bp random library with flanking sequence; DNA aptamer

DAL-N-20 100 ug
EUR 343.2

30 bp random library with flanking sequence; DNA aptamer

DAL-N-30 100 ug
EUR 416.4

40 bp random library with flanking sequence; DNA aptamer

DAL-N-40 100 ug
EUR 489.6

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

20-abx217653
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  • 100 ug
  • 50 ug

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx217653-100tests 100 tests Ask for price

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx217653-200tests 200 tests Ask for price

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx217653-20tests 20 tests
EUR 87.5

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx375543-96tests 96 tests
EUR 337.5

Paxbp1 (untagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), (10ug)

MC222591 10 µg Ask for price

Paxbp1 (GFP-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), (10ug)

MG222463 10 µg Ask for price

Paxbp1 (Myc-DDK-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1)

MR222463 10 µg Ask for price

Cell Cycle Assay Kit

DLSM0247 100 Assays Ask for price
Description: Detection cell cycle and cell proliferation using flow cytometry.

Cell Cycle Assay Kit

abx298909-100g 100 µg Ask for price

Cell Cycle Assay Kit

abx298909-20g 20 µg
EUR 212.5

Cell Cycle Assay Kit

abx298909-50g 50 µg Ask for price

Cell Cycle Sampler Kit

E051008 5x50ug
EUR 475
Description: Available in various conjugation types.

Cell Cycle Staining Kit

KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-50T 50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-each each Ask for price

PAXBP1 (untagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

SC314763 10 µg Ask for price

PAXBP1 (untagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

SC314917 10 µg Ask for price

Lenti ORF clone of Paxbp1 (mGFP-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1)

MR222463L4 10 µg Ask for price

Lenti ORF clone of Paxbp1 (Myc-DDK-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1)

MR222463L3 10 µg Ask for price

PAXBP1 (GFP-tagged) - Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RG224365 10 µg Ask for price

PAXBP1 (GFP-tagged) - Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RG224419 10 µg Ask for price

ACES™ Signal Sequence Kit

0148-2 kit
EUR 198.7

GCFC2 (untagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 2

SC331408 10 µg Ask for price

GCFC2 (untagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 3

SC331409 10 µg Ask for price

GCFC2 (GFP-tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 2

RG234563 10 µg Ask for price

GCFC2 (GFP-tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 3

RG233464 10 µg Ask for price

PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RC224365 10 µg Ask for price

PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RC224419 10 µg Ask for price

GCFC2 (Myc-DDK tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 2

RC234563 10 µg Ask for price

GCFC2 (Myc-DDK tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 3

RC233464 10 µg Ask for price

EZCellTM Cell Cycle Analysis Kit

K920-100 each
EUR 451.2

Lenti ORF particles, Paxbp1 (GFP-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), 200ul, >10^7 TU/mL

MR222463L4V 200 µl Ask for price

NSG1 (untagged)-Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 1

SC115028 10 µg Ask for price

NSG1 (untagged)-Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 2

SC310958 10 µg Ask for price

NSG1 (GFP-tagged) - Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 2

RG206927 10 µg Ask for price

NSG1 (GFP-tagged) - Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 1

RG222554 10 µg Ask for price

Lenti ORF particles, Paxbp1 (Myc-DDK-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), 200ul, >10^7 TU/mL

MR222463L3V 200 µl Ask for price

Cell Cycle Phosphorylation Sampler Kit

E051007 5x50ug
EUR 655
Description: Available in various conjugation types.

Grsf1 ELISA Kit| Mouse G-rich sequence factor 1 ELISA Kit

EF015097 96 Tests
EUR 826.8

Lenti-ORF clone of PAXBP1 (mGFP-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RC224365L4 10 µg Ask for price

Lenti-ORF clone of PAXBP1 (mGFP-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RC224419L4 10 µg Ask for price

NSG1 (Myc-DDK-tagged)-Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 2

RC206927 10 µg Ask for price

NSG1 (Myc-DDK-tagged)-Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 1

RC222554 10 µg Ask for price

Mouse G- rich sequence factor 1, Grsf1 ELISA KIT

ELI-42875m 96 Tests
EUR 1038

Human G- rich sequence factor 1, GRSF1 ELISA KIT

ELI-39072h 96 Tests
EUR 988.8

Cell Cycle Assay Kit (Red Fluorescence)

E-CK-A351-100Assays 100 Assays
EUR 128

Cell Cycle Assay Kit (Red Fluorescence)

E-CK-A351-20Assays 20 Assays
EUR 45

Cell Cycle Assay Kit (Red Fluorescence)

E-CK-A351-50Assays 50 Assays
EUR 78

Cell Cycle Assay Kit (Red Fluorescence)

E-CK-A351-each each Ask for price
Description: Cell Cycle

Human G-Rich Sequence Factor 1 (GRSF1) ELISA Kit

abx384974-96tests 96 tests
EUR 1093.2

Mouse G-Rich Sequence Factor 1 (GRSF1) ELISA Kit

abx389463-96tests 96 tests
EUR 1093.2

SFRS17A (untagged)-Human DNA segment on chromosome X and Y (unique) 155 expressed sequence (cDNA clone MGC:39904 IMAGE:5217247), complete cds

SC123528 10 µg Ask for price

Akap17a (untagged ORF) - Rat similar to DNA segment on chromosome X and Y (unique) 155 expressed sequence isoform 1 (LOC288526), (10 ug)

RN204888 10 µg Ask for price

Cell Cycle Assay Kit (Blue Fluorescence)

E-CK-A353-100Assays 100Assays
EUR 128
Description: Cell Cycle

Cell Cycle Assay Kit (Blue Fluorescence)

E-CK-A353-20Assays 20Assays
EUR 45
Description: Cell Cycle

Cell Cycle Assay Kit (Blue Fluorescence)

E-CK-A353-50Assays 50Assays
EUR 78
Description: Cell Cycle

Cell Cycle Assay Kit (Blue Fluorescence)

E-CK-A353-each each Ask for price
Description: Cell Cycle

Lenti-ORF clone of PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RC224365L3 10 µg Ask for price

Lenti-ORF clone of PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RC224419L3 10 µg Ask for price

Lenti ORF clone of Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 2, mGFP tagged

RC206927L4 10 µg Ask for price

Lenti ORF clone of Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 1, mGFP tagged

RC222554L2 10 µg Ask for price

Lenti ORF clone of Human DNA segment on chromosome 4 (unique) 234 expressed sequence (D4S234E), transcript variant 1, mGFP tagged

RC222554L4 10 µg Ask for price

Cell Cycle Assay Kit (Green Fluorescence)

E-CK-A352-100Assays 100Assays
EUR 128
Description: Cell Cycle

Cell Cycle Assay Kit (Green Fluorescence)

E-CK-A352-20Assays 20Assays
EUR 45
Description: Cell Cycle

Cell Cycle Assay Kit (Green Fluorescence)

E-CK-A352-50Assays 50Assays
EUR 78
Description: Cell Cycle

Cell Cycle Assay Kit (Green Fluorescence)

E-CK-A352-each each Ask for price
Description: Cell Cycle

human similar to cDNA sequence BC027382 ELISA Kit

201-12-1889 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human similar to cDNA sequence BC027382 ELISA Kit

GA-E1905HM-48T 48T
EUR 346.8

Human similar to cDNA sequence BC027382 ELISA Kit

GA-E1905HM-96T 96T
EUR 559.2

Human similar to cDNA sequence BC027382 ELISA Kit

QY-E02390 96T
EUR 433.2

human similar to cDNA sequence BC027382 ELISA Kit

YLA2833HU-48T 48T Ask for price

human similar to cDNA sequence BC027382 ELISA Kit

YLA2833HU-96T 96T Ask for price

Single Cell Sequence Specific Amplification Kit

P621-01 200 rxn
EUR 435

Rat Cell Division Cycle 23 ELISA kit

E01A10996 96T
EUR 700
Description: ELISA

Rat Cell Division Cycle 23 ELISA kit

E02C0478-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cell Division Cycle 23 ELISA kit

E02C0478-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cell Division Cycle 23 ELISA kit

E02C0478-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cell Division Cycle 23 ELISA kit

E08C0478-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cell Division Cycle 23 ELISA kit

E08C0478-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cell Division Cycle 23 ELISA kit

E08C0478-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cell Division Cycle 23 ELISA kit

E07C0478-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cell Division Cycle 23 ELISA kit

E07C0478-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cell Division Cycle 23 ELISA kit

E07C0478-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cell Division Cycle 23 ELISA kit

E01A45908 96T
EUR 700
Description: ELISA

Goat Cell Division Cycle 23 ELISA kit

E06C0478-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cell Division Cycle 23 ELISA kit

E06C0478-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cell Division Cycle 23 ELISA kit

E06C0478-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Cell Division Cycle 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat cell division cycle (CDC) ELISA Kit

NSL1569r 96 Tests
EUR 498

Lenti ORF particles, PAXBP1 (mGFP-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1, 200ul, >10^7 TU/mL

RC224365L4V 200 µl Ask for price

Transcription issue binding website motif scanning recognized C2H2 ZF, AP-2 and CxxC as probably components practical on the promoters of down-regulated genes concerned in calcium signaling and vascular easy muscle contraction. As well as, 1686 genes exhibited various splicing that had been primarily implicated in microtubule group and easy muscle contraction. Cross-comparison evaluation recognized novel genes that had been each differentially expressed and alternatively spliced; these had been concerned in leukocyte and B cell biology and lipid metabolism. In conclusion, this primary complete research offers a beneficial useful resource for understanding the molecular mechanism underlying gestational uterine arterial diversifications throughout being pregnant.

 

Evaluation of a fully automated bioinformatics tool to predict antibiotic resistance from MRSA genomes.

Evaluation of a fully automated bioinformatics tool to predict antibiotic resistance from MRSA genomes.

The genetic prediction of phenotypic antibiotic resistance primarily based on evaluation of WGS information is turning into more and more possible, however a significant barrier to its introduction into routine use is the shortage of absolutely automated interpretation instruments. Right here, we report the findings of a big analysis of the Subsequent Gen Diagnostics (NGD) automated bioinformatics evaluation software to foretell the phenotypic resistance of MRSA.MRSA-positive sufferers had been recognized in a medical microbiology laboratory in England between January and November 2018. One MRSA isolate per affected person along with all blood tradition isolates (whole n = 778) had been sequenced on the Illumina MiniSeq instrument in batches of 21 medical MRSA isolates and three controls.The NGD system activated post-sequencing and processed the sequences to find out inclined/resistant predictions for 11 antibiotics, taking round 11 minutes to analyse 24 isolates sequenced on a single sequencing run.

NGD outcomes had been in contrast with phenotypic susceptibility testing carried out by the medical laboratory utilizing the disc diffusion methodology and EUCAST breakpoints. Following retesting of discrepant outcomes, concordance between phenotypic outcomes and NGD genetic predictions was 99.69%.  Genetic predictions generated by the NGD software had been in contrast with predictions generated by an unbiased research-based informatics method, which demonstrated an total concordance between the 2 strategies of 99.97%.We conclude that the NGD system supplies speedy and correct prediction of the antibiotic susceptibility of MRSA.

Streamlining DNA Sequencing and Bioinformatics Evaluation Utilizing Software program Containers.

Advances in software program containerization are revolutionizing the way in which functions are distributed and executed. Containers are stand-alone software program environments that encapsulate all dependencies an software may have, are constructed from well-defined recipes, and are immutable and moveable, making certain reliability and reproducibility of outcomes. The Bioinformatics Core of the Interdisciplinary Middle for Biotechnology Analysis (ICBR) is utilizing containers to streamline the administration of SubsequentGen Sequencing (NGS) information generated by the middle’s Sequencing Core. NGS information evaluation often begins with a sequence of quality-control and cleanup steps which can be frequent to most functions.

These embrace trimming reads on the premise of high quality, producing reviews, and producing primary statistics on the sequencing run output (e.g. variety of reads per pattern, fraction of low-quality reads, and so forth). These preliminary steps have been containerized and are actually executed robotically after every sequencing run, earlier than the datasets are handed over to the Bioinformatics Core for evaluation. This technique presents three benefits. Additional investigation of 22 isolate genomes related to persistent discrepancies revealed a spread of causes in 12 circumstances, however no trigger may very well be discovered for the rest.

First, QC reviews are instantly accessible after the sequencing run is full and could be delivered to the shopper instantly. Second, any issues with the info could be detected, and if obligatory addressed, earlier than beginning the evaluation, saving valuable time. Third, Bioinformatics Core workers are free of having to carry out these routine duties and are capable of concentrate on the precise evaluation of the info. We describe the implementation of the containers, and the way they had been built-in into the usual workflow of the sequencing core. Examples embrace era of QC reviews through FASTQC and MULTIQC in addition to learn trimming through Trimmomatic or fastp. We additionally report on a preliminary analysis of the advantages by way of quicker undertaking turnaround and buyer suggestions. Future plans embrace integration with CrossLabs, utilizing customized kinds to pick the precise pre-processing steps to be carried out after every sequencing run.

Evaluation of a fully automated bioinformatics tool to predict antibiotic resistance from MRSA genomes.

Automated Unbiased Metagenomic DNA Extraction for Lengthy-Learn Sequencing.

Lengthy-read DNA sequencing is being touted because the subsequent subsequentgen sequencing as a consequence of its affordability, ease of use, and elevated output of extra correct information in comparison with conventional subsequentgeneration sequencing applied sciences. For instance, some great benefits of Nanopore long-read sequencing embrace the capability to generate very lengthy reads with exceptional pace and portability, spanning tandem-repeat areas, which resolves ambiguity throughout genome meeting. Nevertheless, extracting inhibitor-free excessive molecular weight (HMW) DNA appropriate for long-read sequencing has all the time been a problem as a consequence of DNA fragmentation throughout extraction brought on by bodily and enzymatic (DNases) breakage.

Right here we current an automatic HMW DNA extraction pipeline that mixes magnetic bead-based DNA extraction with the Microlab STAR liquid handler. Magnetic bead chemistry permits automated processing by retaining HMW DNA throughout stringent wash steps, resulting in excessive portions of long-read sequencing-ready DNA. To check the efficacy of this automated workflow, HMW DNA was remoted from a well-defined combination of micro organism and yeast cells and subsequently sequenced utilizing the MinION platform. Utilizing this methodology, we achieved 1M reads, eight Gb throughput, with common read-lengths of eight kb, and over 100 kb recorded.

Furthermore, we discover that the extracted microbial profile and proportional composition intently matches the theoretical composition. General, we’ve developed an automatic methodology for HMW DNA extraction that reveals unbiased microbial lysis that’s appropriate with long-read nanopore sequencing. Bacterial floor show libraries are a preferred software for novel ligand discovery as a consequence of their ease of manipulation and speedy progress charges. These libraries usually categorical a scaffold protein embedded inside the outer membrane with a brief, surface-exposed peptide that’s both terminal or is included into an outer loop, and may due to this fact work together with and bind to substrates of curiosity.

On this examine, we employed a novel bacterial peptide show library which includes brief 15-mer peptides on the floor of E. coli, co-expressed with the inducible crimson fluorescent protein DsRed within the cytosol, to research inhabitants variety over two rounds of biopanning. The naive library was utilized in panning trials to pick for binding affinity towards 3D printing plastic coupons constructed from polylactic acid (PLA).

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 174

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 262.8

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1988.4

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 781.2

Sequencing Grade V8 Protease

RE014 50ug
EUR 209

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 375.6

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 343.2

Sequencing Grade Modified Trypsin

RE012 100ug
EUR 229

Sequencing Grade Carboxypeptidase B

RE011 100ug
EUR 99

ACES Sequencing Buffer 10X, pH 7.7

21420000-1 1 L
EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7

21420000-2 4 L
EUR 456.17

TBE-Buffer 5X for DNA Sequencing

40121072-1 100 mL
EUR 26.54

TBE-Buffer 5X for DNA Sequencing

40121072-2 500 mL
EUR 44.84

TBE-Buffer 5X for DNA Sequencing

40121072-3 1 L
EUR 72.29

TBE-Buffer 5X for DNA Sequencing

40121072-4 4 L
EUR 192.14

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ100UG each
EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ1MG each
EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ400UG each
EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

17068 5 nmoles
EUR 2091

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056
  • Ask for price
  • Ask for price
  • Ask for price
  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 576

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY100UG each
EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY1MG each
EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY400UG each
EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

FlashBAC - Baculovirus Expression System (96 reactions)

GWB-6C34E3 Kit Ask for price

FlashBAC - Baculovirus Expression System (5 reactions)

GWB-7676BB Kit Ask for price

FlashBAC - Baculovirus Expression System(24 reactions)

GWB-EB1FF8 Kit Ask for price

FlashBAC Gold - Baculovirus Expression System (5 reactions)

GWB-97BE6D Kit Ask for price

FlashBAC Gold - Baculovirus Expression System(24 reactions)

GWB-67B0AE Kit Ask for price

FlashBAC Gold - Baculovirus Expression System (96 reactions)

GWB-FB2AFE Kit Ask for price

flashBAC Ultra - Baculovirus Expression System (5 reactions)

GWB-6AB80F Kit Ask for price

flashBAC Ultra - Baculovirus Expression System (24 reactions)

GWB-DC7364 Kit Ask for price

Amersham Rediprime II DNA Labeling System 30 reactions - EACH

RPN1633 EACH
EUR 832.95

Genorise® Customized PCR primers for cloning and sequencing

GR108023 2 nmol
EUR 65

Wizard Block Bundle Special (Wizard: Classic 1/2, 3/4 & Wizard: Cryo 1/2)

M-R-1008657 -
EUR 914
Description: Wizard Block Bundle Special (Wizard: Classic 1/2, 3/4 & Wizard: Cryo 1/2)

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

IBOCHYTLCKSG100UG each
EUR 297
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

Tube Bundle Special (Wizard: Classic 1,2,3,4; Wizard: Cryo 1,2)

M-R-10095402 288 X 10 ml
EUR 2070
Description: Tube Bundle Special (Wizard: Classic 1,2,3,4; Wizard: Cryo 1,2)

GWB-97BE6D-1U - FlashBAC Gold - Baculovirus Expression System (5 reactions)

GWB-97BE6D-1U 5reactions
EUR 763

GWB-67B0AE-1U - FlashBAC Gold - Baculovirus Expression System(24 reactions)

GWB-67B0AE-1U 24reactions
EUR 2044

GWB-6AB80F-1U - flashBAC Ultra - Baculovirus Expression System (5 reactions)

GWB-6AB80F-1U 5reactions
EUR 863

GWB-DC7364-1U - flashBAC Ultra - Baculovirus Expression System (24 reactions)

GWB-DC7364-1U 24reactions
EUR 2411

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

IBCTCLOSGPF10UG each
EUR 165
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY250UG each
EUR 784
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY50UG each
EUR 304
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

JBScreen Wizard 1

CS-311 48solutions
EUR 435.6

JBScreen Wizard 2

CS-312 48solutions
EUR 435.6

JBScreen Wizard 1 & 2

CS-315 2Kits
EUR 826.9

JBScreen Wizard 1 & 2 HTS

CS-318 96solutions
EUR 329.4

3M Clean-Trace Hygiene Monitoring and Management System - EACH

FSA1260 EACH
EUR 7275.15

Rigaku Wizard Cubic LCP Kit

MLCP-RWCK-1 1 KIT
EUR 2070
Description: Rigaku Wizard Cubic LCP Kit

Rigaku Wizard Cubic LCP Screen

MLCP-RWCS-1 1 KIT
EUR 316
Description: Rigaku Wizard Cubic LCP Screen

2X Reaction Buffer

1068-20 each
EUR 222

2X Reaction Buffer

1068-80 each
EUR 352.8

TDO Reaction Solution

73005 10 ml
EUR 205
Description: TDO Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for TDO-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS TDO Inhibitor Screening Assay Kit (BPS Cat. #72023) and/or with purified, active TDO, His-tag (BPS Cat. #71195) in combination with 1x TDO Assay Buffer (BPS Cat. #73006).

IDO1 Reaction Solution

73001 10 ml
EUR 205
Description: IDO1 Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO1-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS IDO1 Inhibitor Screening Assay Kit (BPS Cat. #72021) and/or with purified, active IDO1, His-tag (BPS Cat. #71182) in combination with 1x IDO1 Assay Buffer (BPS Cat. #73002).

IDO2 Reaction Solution

73003 10 ml
EUR 205
Description: IDO2 Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO2-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS IDO2 Inhibitor Screening Assay Kit (BPS Cat. #72022) and/or with purified, active IDO2, His-tag (BPS Cat. #71194) in combination with 1x IDO2 Assay Buffer (BPS Cat. #73004).

Individual Reaction Mix

G065-X 200 reactions
EUR 200.4

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

Taq Reaction Buffer 10X

11170008-1 5 mL Ask for price

Poly (A) Reaction Buffer

11170058-1 10 mL
EUR 34.4

Pfu Reaction Buffer 10X

11170070-1 5x1 ml
EUR 27.17

MyFi Reaction Buffer, 5x

BIO-37115 3 x 1.25ml Ask for price

Taq Reaction Buffer - Red

abx461046-1096tests 10 × 96 tests Ask for price

Taq Reaction Buffer - Red

abx461046-596tests 5 × 96 tests Ask for price

Taq Reaction Buffer - Red

abx461046-96tests 96 tests
EUR 500

RANGER Reaction Buffer, 5x

BIO-37114 3 x 1.25ml Ask for price

Reaction Columns 2ml - PK100

CHR5348 PK100
EUR 229.5

flashBAC ULTRA (96 reaction)

GWB-1C1F25 96 reactions Ask for price

Individual Reaction Mix 1

G065-1 200 reactions
EUR 200.4

Individual Reaction Mix 10

G065-10 200 reactions
EUR 200.4

Individual Reaction Mix 11

G065-11 200 reactions
EUR 200.4

Individual Reaction Mix 12

G065-12 200 reactions
EUR 200.4

Individual Reaction Mix 2

G065-2 200 reactions
EUR 200.4

Individual Reaction Mix 3

G065-3 200 reactions
EUR 200.4

Individual Reaction Mix 4

G065-4 200 reactions
EUR 200.4

Individual Reaction Mix 5

G065-5 200 reactions
EUR 200.4

Individual Reaction Mix 6

G065-6 200 reactions
EUR 200.4

Individual Reaction Mix 7

G065-7 200 reactions
EUR 200.4

Individual Reaction Mix 8

G065-8 200 reactions
EUR 200.4

Individual Reaction Mix 9

G065-9 200 reactions
EUR 200.4

ReadiUseâ„¢ PCR Reaction Buffer

17202 50mL
EUR 97
Description: PCR (polymerase chain reaction) is a laboratory technique used to amplify DNA (deoxyribonucleic acid) segments.

ReadiUseâ„¢ PCR Reaction Buffer

17202-50mL 50 mL
EUR 95
Description: Our ready to use PCR buffer contains several components essential for the PCR reaction. The PCR reaction buffer helps to maintain a stable pH during the PCR reaction. It also contains a salt that helps to maintain the integrity of the DNA during the reaction and also enhances the activity of the DNA polymerase enzyme.

amfiXpand 2x Reaction Buffer

P0333-001 1ml
EUR 121.2

amfiFusion 2x Reaction Buffer

P0343-001 1ml
EUR 124.8

Taq Reaction Buffer - Colorless

abx461045-1096tests 10 × 96 tests Ask for price

Taq Reaction Buffer - Colorless

abx461045-596tests 5 × 96 tests Ask for price

Taq Reaction Buffer - Colorless

abx461045-96tests 96 tests
EUR 437.5

Hi-Fi Reaction Buffer, 5x

BIO-37110 2 x 1.5ml Ask for price

DNA Polymerase Reaction Buffer

abx461036-1096tests 10 × 96 tests Ask for price

DNA Polymerase Reaction Buffer

abx461036-596tests 5 × 96 tests Ask for price

DNA Polymerase Reaction Buffer

abx461036-96tests 96 tests
EUR 562.5

e-Taq Reaction Buffer 10X

11170006-1 5 mL Ask for price

F-Taq Reaction Buffer 10X

11170007-1 5 mL Ask for price
Description: FastStart Taq DNA Polymerase

h-Taq Reaction Buffer 10X

11170029-1 5 mL Ask for price

Templiphi 2000 Reaction Kit - EACH

28964286 EACH
EUR 5000.4

MyTaq Reaction Buffer Red, 5x

BIO-37112 4 x 1ml Ask for price

genesig q16 reaction tubes, 54

Z-genesigEASY-0.2 54 tubes
EUR 17

TDO Fluorogenic Reaction Solution

73010 10 ml
EUR 205
Description: TDO Fluorogenic Reaction Solution is an optimized, proprietary_x000D_formulation containing all the necessary reaction components for TDO-catalyzed_x000D_conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays._x000D_This product is intended to be used with the BPS Human TDO Fluorogenic Inhibitor_x000D_Screening Assay Kits (BPS Cat. #72039 & Cat. #72049) and/or with purified, active TDO,_x000D_His-tag (BPS Cat. #71195) in combination with 1x TDO Assay Buffer (BPS Cat. #73006).

amfiEco Taq Reaction Buffer(2X)

P0703-001 1ml
EUR 100.8

amfiEco Taq Reaction Buffer(2X)

P0703-005 5ml
EUR 213.6

IDO1 Fluorogenic Reaction Solution

73009 10 ml
EUR 205
Description: IDO1 Fluorogenic Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO1-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS Human IDO1 Fluorogenic Inhibitor Screening Assay Kit (BPS Cat. #72037 &Cat. #72047) and/or with purified, active IDO1, His-tag (BPS Cat. #71182) in combination with 1x IDO1 Assay Buffer (BPS Cat. #73002).

DiaStar Taq Reaction Buffer 10X

11170005-1 5 mL Ask for price

48 reaction RT premix with nanoscript 2, 48 reactions

Z-RT-premix2-48 48 tests
EUR 424

MyTaq reaction Buffer Colourless, 5x

BIO-37111 4 x 1ml Ask for price

Wide Neck Flask Reaction 700ml Quickfit - PK5

QFR700F0 PK5
EUR 945

Wide Neck Flask Reaction 250ml Quickfit - EACH

QFR250F EACH
EUR 112.05

Wide Neck Flask Reaction 500ml Quickfit - EACH

QFR500F EACH
EUR 129.6

Pfu Reaction Buffer with MgSO4 10X

11170071-1 5x1 mL
EUR 27.28

Kinase Reaction Buffer Concentrate, 60ML

X104-60ML 60ML
EUR 59

2X Tranzyme MasterMix (BP) Reaction kit

E36E082 50μl/20 reaction
EUR 192.86

2X Tranzyme MasterMix (BP) Reaction kit

E36E083 250μl/100 reaction
EUR 972.86

genesigEASY q16 0.1ml reaction tubes, 1000

A00001-1K 1000 tubes
EUR 151

GFP-nAb Magnetic Kit (20 Reactions)

ABP-NAB-GFPMK20 20 Reactions Ask for price

RFP-nAb Magnetic Kit (20 Reactions)

ABP-NAB-RFPMK20 20 Reactions Ask for price

Wizard Cryo 1 Tubes (48 formulations 10 mL volumes)

M-R-1009536 48 X 10 ml
EUR 374
Description: Wizard Cryo 1 Tubes (48 formulations 10 mL volumes)

Wizard Cryo 2 Tubes (48 formulations 10 mL volumes)

M-R-1009537 48 X 10 ml
EUR 374
Description: Wizard Cryo 2 Tubes (48 formulations 10 mL volumes)

Wizard Classic 1 Tubes (48 formulations 10 mL volumes)

M-R-1009530 48 X 10 ml
EUR 374
Description: Wizard Classic 1 Tubes (48 formulations 10 mL volumes)

Wizard Classic 2 Tubes (48 formulations 10 mL volumes)

M-R-1009531 49 X 10 ml
EUR 374
Description: Wizard Classic 2 Tubes (48 formulations 10 mL volumes)

Wizard Classic 3 Tubes (48 formulations 10 mL volumes)

M-R-1009533 48 X 10 ml
EUR 374
Description: Wizard Classic 3 Tubes (48 formulations 10 mL volumes)

Wizard Classic 4 Tubes (48 formulations 10 mL volumes)

M-R-1009534 48 X 10 ml
EUR 374
Description: Wizard Classic 4 Tubes (48 formulations 10 mL volumes)

C Reaction Protein (CRP) Antibody-BIOTIN

E64C00503 100ug/100ul
EUR 275
Description: Available in various conjugation types.

Ensuing libraries had been then deep-sequenced utilizing subsequent era sequencing (NGS) to research choice and variety.We demonstrated enrichment for PLA binding versus a sapphire management floor, analyzed inhabitants composition, and in contrast sorting rounds utilizing a binding assay and fluorescence microscopy. The potential to supply and describe show libraries via NGS throughout rounds of choice permits a deeper understanding of inhabitants dynamics that may be higher directed in direction of peptide discovery.

 

TIAs pathway genes and associated miRNA identification in Vinca minor: supporting aspidosperma and eburnamine alkaloids linkage via transcriptomic analysis

TIAs pathway genes and associated miRNA identification in Vinca minor: supporting aspidosperma and eburnamine alkaloids linkage via transcriptomic analysis

V. minor incorporates monomeric eburnamine-type of indole alkaloids having utilization as a neuro-medicinal plant. The biosynthetic pathway analysis using miRNAs has been the focal point for plant genomic evaluation these days and this technique is utilized to get an notion proper into a possible pathway diploma look at in V. minor as understanding of genes on this prized medicinal plant is meagrely understood. The de novo transcriptomic analysis using Illumina Subsequent gen sequencing has been carried out in glasshouse shifted plant and transformed roots to elucidate the potential non confirmed steps of terpenoid indole alkaloids (TIAs) pathway in V. minor

. A putative TIA pathway is elucidated inside the look at collectively with twelve potential TIAs biosynthetic genes. The actual miRNA associated to TIAs pathway have been acknowledged and their roles have been talked about for the first time in V. minor. The comparative analysis of transcriptomic data of glasshouse shifted plant and transformed roots confirmed that the raw reads of transformed roots have been bigger (83,740,316) as compared with glasshouse shifted plant (67,733,538). The EST-SSR prediction confirmed the utmost widespread repeats amongst glasshouse shifted plant and transformed roots, although small variation was current in trinucleotide repeats restricted to glasshouse shifted plant. The look at reveals complete 37 miRNAs which have been observed to be true and should have a process in pathway as they’re going to regulate the enlargement and alkaloid manufacturing. The identification of putative pathway genes performs an important operate in establishing linkage between Aspidosperma and Eburnamine alkaloids.

Groundwater-N air air pollution derives from agricultural and concrete actions, and compromises water top quality in shallow aquifers, inserting human and environmental properly being at risk. Nonetheless, subsurface microbiota can transform dissolved inorganic nitrogen into N2. On this look at, we surveyed the microbial group of a shallow aquifer by sampling one successfully, one piezometer and a spring inside an agricultural area that receives N-inputs of larger than 700 kg/ha per yr by way of irrigation with wastewater.

The survey was carried out all through a yr with a 16S rRNA subsequentgen technique. In parallel, we quantified the number of gene copies and transcripts related to anaerobic ammonium oxidation (anammox, hzo), nitrite-dependent anaerobic methane oxidation (n-damo, nod and pmoA) and nitrous oxide low cost (last step of denitrification, nosZ), by way of the dry and moist seasons. Our outcomes confirmed that the groundwater samples had 17.7 to 22.5 mg/L of NO3-N. The bacterial and archaeal group development was distinctive at each web site, and it remained comparatively regular over time. We verified the co-occurrence of N-transforming micro organism, which was correlated with the main target of NO2/NO3 and ORP/DO values (DO: ~3.Zero mg/L).

Near Precise-Time Identification of Newest HIV Transmissions, Transmitted Drug Resistance Mutations, and Transmission Networks by MPID-NGS in North Carolina

The identification of newest HIV-1 infections amongst of us with new diagnoses is crucial to every tailoring and assessing the impression of HIV-1 prevention strategies. We developed a Multiplexed Primer ID-Subsequent Gen Sequencing (MPID-NGS) technique to determine newest infections by measuring the intra-host viral selection over numerous areas of the HIV-1 genome, together with detecting drug resistance mutations (DRMs) and phylogenetically linked clusters. We summarize the sphere implementation of this all-in-one platform amongst people with newly acknowledged HIV-1 by the North Carolina State Laboratory of Public Effectively being in 2018.
 Complete, newest an an infection was acknowledged in 94 (35%) of 268 new diagnoses. Of us youthful than 30 years outdated and people who inject medicine have been further extra prone to be acknowledged as newest an an infection. The RT space Okay103N was most likely essentially the most usually detected DRM (roughly 15%). We found an entire of 28 clusters, and people with newest an an infection have been further extra prone to be cluster members as compared with these with continuous infections (p = 0.026). We present the quick identification of newest an an infection and pre-treatment DRMs coupled with cluster analysis that may allow prioritization of linkage to care, remedy and prevention interventions to those at highest risk of onward transmission.
TIAs pathway genes and associated miRNA identification in Vinca minor: supporting aspidosperma and eburnamine alkaloids linkage via transcriptomic analysis

Fenotipo de hipercolesterolemia acquainted definitivo con estudio genético negativo en Argentina

We developed public web sites and assets for knowledge entry, show, and evaluation of plant small RNAs. These web sites are interconnected with associated knowledge sorts. The present technology of those informatics instruments was developed for Illumina knowledge, evolving over >15 years of enhancements. Our on-line databases have custom-made net interfaces to uniquely deal with and show RNA-derived knowledge from numerous plant species, starting from Arabidopsis thaliana to wheat (Triticum sp.), together with many crop and mannequin species. The net interface shows the abundance and genomic context of knowledge for small RNAs, parallel evaluation of RNA ends (PARE)/degradome reads, RNA-seq, and even chromatin immunoprecipitation (ChIP)-seq knowledge; it additionally supplies details about doubtlessly novel transcripts (antisense transcripts, various splice isoforms, regulatory intergenic transcripts).

SequaGel MD SSCP Kit - 1KIT

NAT1170 1KIT
EUR 318.6

sequencing unit 33x45 cm

ESEQ1100-SYS ea
EUR 1842

450ML SequaGel XR - 450ML

NAT1160 450ML
EUR 112.05

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 174

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 262.8

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1988.4

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 781.2

Sequencing Grade Chymotrypsin

RE013 100ug
EUR 309

1L SequaGel XR - 1L

NAT1162 1L
EUR 175.5

Sequencing Grade V8 Protease

RE014 50ug
EUR 209

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056
  • Ask for price
  • Ask for price
  • Ask for price
  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

SequaGel MD Heteroduplex Kit - EACH

NAT1172 EACH
EUR 337.5

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 375.6

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 343.2

Sequencing Grade Modified Trypsin

RE012 100ug
EUR 229

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 114.82

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 553.3

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 576

ACES Sequencing Buffer 10X, pH 7.7

21420000-1 1 L
EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7

21420000-2 4 L
EUR 456.17

Sequencing Grade Carboxypeptidase B

RE011 100ug
EUR 99

TBE-Buffer 5X for DNA Sequencing

40121072-1 100 mL
EUR 26.54

TBE-Buffer 5X for DNA Sequencing

40121072-2 500 mL
EUR 44.84

TBE-Buffer 5X for DNA Sequencing

40121072-3 1 L
EUR 72.29

TBE-Buffer 5X for DNA Sequencing

40121072-4 4 L
EUR 192.14

100ML SequaGel Buffer - 100ML

NAT1140 100ML
EUR 36.45

1L SequaGel UreaGel-6 - 1L

NAT1146 1L
EUR 152.55

1L SequaGel UreaGel-8 - 1L

NAT1150 1L
EUR 152.55

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ100UG each
EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ1MG each
EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ400UG each
EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

17068 5 nmoles
EUR 2091

1L SequaGel UreaGel Diluent - 1L

NAT1154 1L
EUR 72.9

450ML SequaGel UreaGel-6 - 450ML

NAT1144 450ML
EUR 95.85

450ML SequaGel UreaGel-8 - 450ML

NAT1148 450ML
EUR 95.85

1L SequaGel UreaGel Concentrate - 1L

NAT1134 1L
EUR 160.65

200ML SequaGel UreaGel Buffer - 200ML

NAT1142 200ML
EUR 41.85

90ML SequaGel Complete Buffer - 90ML

NAT1156 90ML
EUR 35.1

100ML SequaGel XR Concentrate - 100ML

NAT1164 100ML
EUR 116.1

450ML SequaGel XR Concentrate - 450ML

NAT1166 450ML
EUR 494.1

450ML SequaGel UreaGel Diluent - 450ML

NAT1152 450ML
EUR 52.65

450ML SequaGel UreaGel Concentrate - 450ML

NAT1132 450ML
EUR 112.05

1.2ML SequaGel MD SSCP Stop Solution - 1.2ML

NAT1174 1.2ML
EUR 44.55

200ML SequaGel MD Monomer Solution - 200ML

NAT1168 200ML
EUR 264.6

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY100UG each
EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY1MG each
EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY400UG each
EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

200ML SequaGel UreaGel Complete Buffer - 200ML

NAT1158 200ML
EUR 44.55

Genorise® Customized PCR primers for cloning and sequencing

GR108023 2 nmol
EUR 65

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

IBOCHYTLCKSG100UG each
EUR 297
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY250UG each
EUR 784
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY50UG each
EUR 304
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

IBCTCLOSGPF10UG each
EUR 165
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

UreaGel 29:1 System 2.2L Kit - EACH

NAT1130 EACH
EUR 318.6

Watershed System Kit

M-WS180320-1 1 KIT
EUR 9296
Description: Watershed System Kit

Cy3 TSA Fluorescence System Kit

K1051-100 100-300 slides
EUR 184
Description: used for signal amplification of in situ hybridization(ISH), immunohistochemistry(IHC), immunocytochemistry(ICC) and other related experiments

Cy3 TSA Fluorescence System Kit

K1051-200 200-600 slides
EUR 340
Description: used for signal amplification of in situ hybridization(ISH), immunohistochemistry(IHC), immunocytochemistry(ICC) and other related experiments

Cy5 TSA Fluorescence System Kit

K1052-100 100-300 slides
EUR 184
Description: used for signal amplification of in situ hybridization(ISH), immunohistochemistry(IHC), immunocytochemistry(ICC) and other related experiments

Cy5 TSA Fluorescence System Kit

K1052-200 200-600 slides
EUR 340
Description: used for signal amplification of in situ hybridization(ISH), immunohistochemistry(IHC), immunocytochemistry(ICC) and other related experiments

ACES™ Signal Sequence Kit

0148-2 kit
EUR 198.7

Fluorescein TSA Fluorescence System Kit

K1050-100 100-300 slides
EUR 184
Description: used for signal amplification of in situ hybridization (ISH), immunohistochemistry (IHC), immunocytochemistry (ICC) and other related experiments

Fluorescein TSA Fluorescence System Kit

K1050-200 200-600 slides
EUR 340
Description: used for signal amplification of in situ hybridization (ISH), immunohistochemistry (IHC), immunocytochemistry (ICC) and other related experiments

UreaGel 29:1 System 1L Kit - EACH

NAT1128 EACH
EUR 187.65

SBA Clonotyping System Kit : HRP (OKSB00003)

OKSB00003 1 Kit
EUR 944.4
Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity:

ProtoBlock System - 1KIT

NAT1004 1KIT
EUR 105.3

Grsf1 ELISA Kit| Mouse G-rich sequence factor 1 ELISA Kit

EF015097 96 Tests
EUR 826.8

Mouse G- rich sequence factor 1, Grsf1 ELISA KIT

ELI-42875m 96 Tests
EUR 1038

Human G- rich sequence factor 1, GRSF1 ELISA KIT

ELI-39072h 96 Tests
EUR 988.8

New system - EACH

VS10WPGS2 EACH
EUR 106.65

OKSB00003-1U - SBA Clonotyping System Kit : HRP

OKSB00003-1U 1kit
EUR 629

Human G-Rich Sequence Factor 1 (GRSF1) ELISA Kit

abx384974-96tests 96 tests
EUR 1093.2

Mouse G-Rich Sequence Factor 1 (GRSF1) ELISA Kit

abx389463-96tests 96 tests
EUR 1093.2

CCD cooling system

3600100 1unit
EUR 434000

NBReader Q3 system

M1001 1set
EUR 900
Description: Instrument

AEC coloring system

CT356 10-plate
EUR 169.2

human similar to cDNA sequence BC027382 ELISA Kit

201-12-1889 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human similar to cDNA sequence BC027382 ELISA Kit

GA-E1905HM-48T 48T
EUR 346.8

Human similar to cDNA sequence BC027382 ELISA Kit

GA-E1905HM-96T 96T
EUR 559.2

Human similar to cDNA sequence BC027382 ELISA Kit

QY-E02390 96T
EUR 433.2

human similar to cDNA sequence BC027382 ELISA Kit

YLA2833HU-48T 48T Ask for price

human similar to cDNA sequence BC027382 ELISA Kit

YLA2833HU-96T 96T Ask for price

TMB substrate system

TBS5021-100ML 100 mL
EUR 69

TMB substrate system

TBS5021-1L 1L
EUR 460

QS710 CASTING SYSTEM

IB51030 (TRAY AND FIXTURE)
EUR 138.18

SBA Clonotyping System-AP Kit (OKSB00002)

OKSB00002 1 Kit
EUR 1015.2
Description: Description of target: ;Species reactivity: ;Application: ELISA, ELISPOT;Assay info: ;Sensitivity:

OVA sequence (323-336)

TP1612-10mg 10mg Ask for price
Description: OVA sequence (323-336)

OVA sequence (323-336)

TP1612-1g 1g Ask for price
Description: OVA sequence (323-336)

OVA sequence (323-336)

TP1612-1mg 1mg Ask for price
Description: OVA sequence (323-336)

OVA sequence (323-336)

TP1612-50mg 50mg Ask for price
Description: OVA sequence (323-336)

OVA sequence (323-336)

TP1612-5mg 5mg Ask for price
Description: OVA sequence (323-336)

MP1015 CASTING SYSTEM

IB53040 (TRAY AND FIXTURE)
EUR 177.89

HR2025 CASTING SYSTEM

IB56040 (TRAY AND FIXTURE)
EUR 224.93

HR2525 CASTING SYSTEM

IB57040 (TRAY AND FIXTURE)
EUR 224.93

MAP Extraction System

TC-9014-100 each
EUR 680

PROTEIN SYSTEM, MAGELIN,

IB92060 REPLACEMENT LID
EUR 83.8

MAGELIN PROTEIN SYSTEM

IB94000 (GLASS PLATES & PRECAST GELS)
EUR 956.79

MAGELIN PROTEIN SYSTEM

IB94300 (PRECAST GELS ONLY)
EUR 580.72

Single Cell Sequence Specific Amplification Kit

P621-01 200 rxn
EUR 435

Mouse GC- rich sequence DNA- binding factor, Tcf9 ELISA KIT

ELI-12525m 96 Tests
EUR 1038

Human GC- rich sequence DNA- binding factor, TCF9 ELISA KIT

ELI-27490h 96 Tests
EUR 988.8

data acquisition system

D230 ea
EUR 2044.8

Cometslides Rack System

4252-040-02 1 Each
EUR 256.56

Fast Mutagenesis System

20-abx098073
  • Ask for price
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  • 10 rxns
  • 20 rxns

Propane Transfer System

M-CEM-37015 1 UNIT
EUR 688
Description: Propane Transfer System

WESTERN TRANSFER SYSTEM,

IB95030 .2uM PVDF MEMBRANE, 10/PACK
EUR 221.2

Fast Mutagenesis System

abx098073-100l 100 µl
EUR 750

Fast Mutagenesis System

abx098073-1ml 1 ml Ask for price

Fast Mutagenesis System

abx098073-200l 200 µl
EUR 850

Human MCL1(Myeloid Cell Leukemia Sequence 1) ELISA Kit

RE2013H-48wells 48 wells
EUR 125.55

Human MCL1(Myeloid Cell Leukemia Sequence 1) ELISA Kit

RE2013H-96wells 96 wells
EUR 179.55

Human Myeloid cell leukemia sequence 1 (MCL1) ELISA Kit

YLA3907HU-48T 48T Ask for price

Human Myeloid cell leukemia sequence 1 (MCL1) ELISA Kit

YLA3907HU-96T 96T Ask for price

Inhalation Exposure System, 120V

099CA4212 each
EUR 44227

mini system 7x8 cm

EHS3100-SYS ea
EUR 524.4

HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 50 Tests
EUR 678
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 150 Tests
EUR 1602
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 500 Tests
EUR 2422.8
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

mini system 8x10 cm

EHS1050-SYS ea
EUR 422.4

NADPH regeneration system

EGQA0055 50 Tests
EUR 225

WELLMARK PIPETTING SYSTEM

WMKAA0001 -
EUR 377.26

WELLMARK PIPETTING SYSTEM

WMKAA0002 ERGONOMIC EASEL
EUR 102.78

WELLMARK PIPETTING SYSTEM

WMKAA0003 W/ERGONOMIC EASEL
EUR 474.85

Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT

ELI-37633m 96 Tests
EUR 1038

Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT

ELI-08155h 96 Tests
EUR 988.8

ELISA kit for Human MCL1 (Myeloid Cell Leukemia Sequence 1)

E-EL-H1734 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human MCL1 (Myeloid Cell Leukemia Sequence 1) in samples from Serum, Plasma, Cell supernatant

Rat MHC class I polypeptide related sequence A ELISA kit

E01A14621 96T
EUR 700
Description: ELISA

Rat MHC class I polypeptide related sequence B ELISA kit

E01A14622 96T
EUR 700
Description: ELISA

Rat Family with sequence similarity 160, member A1 ELISA kit

E02F0008-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Family with sequence similarity 160, member A1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Family with sequence similarity 160, member A1 ELISA kit

E02F0008-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Family with sequence similarity 160, member A1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Family with sequence similarity 160, member A1 ELISA kit

E02F0008-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Family with sequence similarity 160, member A1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Family With Sequence Similarity 3, Member B ELISA kit

E02F0270-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Family With Sequence Similarity 3, Member B in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Family With Sequence Similarity 3, Member B ELISA kit

E02F0270-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Family With Sequence Similarity 3, Member B in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Family With Sequence Similarity 3, Member B ELISA kit

E02F0270-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Family With Sequence Similarity 3, Member B in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat MHC class I polypeptide related sequence A ELISA kit

E02M0274-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat MHC class I polypeptide related sequence A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat MHC class I polypeptide related sequence A ELISA kit

E02M0274-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat MHC class I polypeptide related sequence A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat MHC class I polypeptide related sequence A ELISA kit

E02M0274-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat MHC class I polypeptide related sequence A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat MHC class I polypeptide related sequence B ELISA kit

E02M0275-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat MHC class I polypeptide related sequence B in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat MHC class I polypeptide related sequence B ELISA kit

E02M0275-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat MHC class I polypeptide related sequence B in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat MHC class I polypeptide related sequence B ELISA kit

E02M0275-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat MHC class I polypeptide related sequence B in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog MHC class I polypeptide related sequence A ELISA kit

E08M0274-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine MHC class I polypeptide related sequence A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog MHC class I polypeptide related sequence A ELISA kit

E08M0274-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine MHC class I polypeptide related sequence A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog MHC class I polypeptide related sequence A ELISA kit

E08M0274-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine MHC class I polypeptide related sequence A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Quite a few choices are included for downloading knowledge as tables or through net providers. Interpretation of those knowledge is facilitated by the inclusion of intensive repeat or transposon knowledge in our genome viewer. We now have developed graphical and analytical instruments, together with a brand new viewer and a question web page for the evaluation of phased small RNAs; these are significantly helpful for understanding the complicated small RNA pathways of vegetation

 

Direct seminal fluid identification by protease-free high-resolution mass spectrometry

Direct seminal fluid identification by protease-free high-resolution mass spectrometry

Serological screening of sexual assault proof has historically targeted on enzyme exercise and immunochromatographic assays that present solely a presumptive indication of seminal fluid and have restricted sensitivity relative to DNA testing. Seminal fluid detection primarily based on protein mass spectrometry represents a “Subsequent Gen” serological expertise that overcomes the specificity and sensitivity limitations of conventional serological screening however requires time-consuming pattern preparation protocols.

This paper describes a novel “peptidomics” strategy to seminal fluid detection that eliminates the necessity for prolonged trypsin digestion. This streamlines pattern preparation to a one-step course of adopted by high-resolution mass spectrometry to establish naturally occurring seminal fluid peptides and low-molecular weight proteins. A number of protein biomarkers of seminal fluid had been persistently and confidently recognized primarily based on the multiplexed detection of quite a few endogenous peptides. This helps the forensic applicability of a peptidomic assay for seminal fluid identification with same-day pattern preparation and evaluation. Our knowledge counsel the predominance of solute service genes throughout metabolic reprogramming of prostate most cancers cells in an androgen-deprived atmosphere, thus signifying them as doubtlessly engaging therapeutic targets.

These included Semenogelin I and II (90% and 86% sequence protection, respectively); Prostate Particular Antigen/p30 (29% sequence protection); and Prostatic Acid Phosphatase (24% sequence protection). The efficiency of this streamlined peptidomics strategy to seminal fluid identification in a forensic context was additionally assessed utilizing simulated casework samples of the kind sometimes collected as a part of a sexual assault examination (e.g., oral and vaginal swabs stained with semen). The ensuing knowledge reveal that sub-microliter portions of seminal fluid on cotton swabs may be recovered and reliably detected. Future improvement and streamlined multiplex peptidomic assays for added organic stains can simply be envisaged.

Metabolic Reprogramming and Predominance of Solute Provider Genes throughout Acquired Enzalutamide Resistance in Prostate Most cancers

Androgen deprivation remedy (ADT) is standard-of-care for advanced-stage prostate most cancers, and enzalutamide (Xtandi®, Astellas, Northbrook, IL, USA), a second technology antiandrogen, is prescribed on this scientific setting. The response to this remedy is normally short-term with the speedy emergence of drug resistance. A greater understanding of gene expression adjustments related to enzalutamide resistance will facilitate circumventing this downside. We in contrast the transcriptomic profile of paired enzalutamide-sensitive and resistant LNCaP and C4-2B prostate most cancers cells for identification of genes concerned in drug resistance by performing an unbiased bioinformatics evaluation and additional validation.

SubsequentGensequencing detected 9409 and 7757 genes differentially expressed in LNCaP and C4-2B cells, in comparison with their parental counterparts. A subset of differentially expressed genes had been validated by qRT-PCR. Evaluation by the i-pathway revealed membrane transporters together with solute service proteins, ATP-binding cassette transporters, and drug metabolizing enzymes as essentially the most distinguished genes dysregulated in resistant cell traces. RNA-Seq knowledge demonstrated predominance of solute service genes SLC12A5, SLC25A17, and SLC27A6 throughout metabolic reprogramming and improvement of drug resistance. Upregulation of those genes had been related to increased uptake of lactic/citric acid and decrease glucose consumption in resistant cells. 

DNA was extracted from the pores and skin swabs and used for subsequent-generation sequencing focusing on the V1-Three area of the 16S rRNA gene. Following a regular microbiota evaluation of the sequencing knowledge, species-level task for the staphylococcal sequences had been obtained utilizing a staphylococci-specific database. Staphylococcus spp. had comparable relative abundance in wholesome and allergic samples. Essentially the most plentiful staphylococcal species had been S. epidermidis in wholesome samples, and S. felis and S. capitis in allergic samples. The composition of staphylococcal communities, in addition to relative abundance of Staphylococcus spp., was variable between physique websites and particular person cats sampled.

Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells

The channel catfish (Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the usual cell line used for channel catfish diagnostics. Subsequentgen sequencing research of a virus cultured within the CCO cells revealed mitochondrial sequences matching these of brown bullhead (Ameiurus nebulosus, Lesueur). Due to this fact, we systematically carried out partial cytochrome oxidase 1 gene sequencing of a number of sources of the CCO cell line and all matched the brown bullhead and never the channel cat. After evaluating the chosen substrates for Prevotella and goal genes of miR-222, these variations urged that responders had been these topics who exhibited impaired glycaemic management. This examine reveals that fecal microbiota and miRNA expression could also be associated to inter-individual variability in scientific trials with polyphenols. This text is protected by copyright. All rights reserved.

Varied Staphylococcus species have been demonstrated to play vital roles on the pores and skin, together with inflicting illness and defending the host from pathogens. Though culture-based research have remoted varied Staphylococcus spp. from feline pores and skin, little or no is understood concerning the species-level communities on the host. To explain the species-level staphylococcal communities inhabiting the pores and skin of wholesome cats and cats with allergic dermatitis. Pores and skin swabs from the ear canal and groin of 11 wholesome and 10 allergic (nonlesional) cats had been obtained. Within the context of the FH detection program in Argentina (Da Vinci Research) 246 hypercholesterolemic sufferers had been evaluated, 21 with DLCN rating > 8 (particular analysis).
Direct seminal fluid identification by protease-free high-resolution mass spectrometry

Phenotype of particular familial hypercholesterolemia with damaging genetic examine in Argentina

Familial hypercholesterolemia (FH) is a monogenic illness, related to variants within the LDLR, APOB and PCSK9 genes. The preliminary analysis relies on scientific standards just like the DLCN standards. A rating > Eight factors qualifies the affected person as “particular” for FH analysis. The detection of the presence of a variant in these genes permits finishing up familial cascade screening and higher characterizes the affected person by way of prognosis and therapy. These sufferers had been studied with subsequent technology sequencing to detect genetic variants, with an prolonged panel of 23 genes; additionally they had been including the big rearrangements evaluation and a polygenic rating of 10 SNP (single nucleotide polymorphism) associated to the rise in LDL-c.

sequencing system 20x50 cm

ESEQ1200-SYS ea
EUR 1842

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 174

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 262.8

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1988.4

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 781.2

Sequencing Grade V8 Protease

RE014 50ug
EUR 209

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 375.6

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 343.2

Sequencing Grade Modified Trypsin

RE012 100ug
EUR 229

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 114.82

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 553.3

Sequencing Grade Carboxypeptidase B

RE011 100ug
EUR 99

ACES Sequencing Buffer 10X, pH 7.7

21420000-1 1 L
EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7

21420000-2 4 L
EUR 456.17

HCV-N-G3/10 (residues 2-119)

PR-1140 100µg
EUR 300.7
Description: recombinant, E. coli

TBE-Buffer 5X for DNA Sequencing

40121072-1 100 mL
EUR 26.54

TBE-Buffer 5X for DNA Sequencing

40121072-2 500 mL
EUR 44.84

TBE-Buffer 5X for DNA Sequencing

40121072-3 1 L
EUR 72.29

TBE-Buffer 5X for DNA Sequencing

40121072-4 4 L
EUR 192.14

SequaGel Sequencing System 1L Kit - 1KIT

NAT1136 1KIT
EUR 187.65

SequaGel Sequencing System 2.2L Kit - EACH

NAT1138 EACH
EUR 318.6

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ100UG each
EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ1MG each
EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ400UG each
EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056
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  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 576

HCV-N-G4 (residues 2-119)

PR-1137 100µg
EUR 300.7
Description: recombinant, E. coli

HCV-N-G5 (residues 2-119)

PR-1138 100µg
EUR 300.7
Description: recombinant, E. coli

HCV-N-G1a (residues 2-119)

PR-1130 100µg
EUR 300.7
Description: recombinant, E. coli

HCV-N-G1b (residues 2-119)

PR-1132 100µg
EUR 300.7
Description: recombinant, E. coli

HCV-N-G2b (residues 2-119)

PR-1134 100µg
EUR 300.7
Description: recombinant, E. coli

HCV-N-G3a (residues 2-119)

PR-1135 100µg
EUR 300.7
Description: recombinant, E. coli

HCV-N-G3b (residues 2-119)

PR-1136 100µg
EUR 300.7
Description: recombinant, E. coli

HCV-N-G6a (residues 2-119)

PR-1139 100µg
EUR 300.7
Description: recombinant, E. coli

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY100UG each
EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY1MG each
EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY400UG each
EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

HCV-N-G2a (residues 2-119)GST

PR-1133 100µg
EUR 300.7
Description: recombinant, E. coli

Genorise® Customized PCR primers for cloning and sequencing

GR108023 2 nmol
EUR 65

RV gE2 (residues 31-105)

PR-1229 100µg
EUR 300.7
Description: recombinant, E. coli

RV-C (residues 1-123)

PR-1230 100µg
EUR 300.7
Description: recombinant, E. coli

TG MIC3 (residues 234-306)

PR-1243 100µg
EUR 300.7
Description: Toxoplasma gondii, recombinant, E. coli

Anti-TAF2(N-terminal-middle residues) Polyclonal Antibody

AB1J129 0.1mg
EUR 399

EBV-EA (residues 306-390)

PR-1224 100µg
EUR 300.7
Description: recombinant, E. coli

CMV pp28 (residues 130-160)

PR-1248 100µg
EUR 300.7
Description: recombinant, E. coli

CMV pp65 (residues 297-510)

PR-1251 100µg
EUR 300.7
Description: recombinant, E. coli

HAV-P2C (residues 1121-1234)

PR-1115 100µg
EUR 300.7
Description: recombinant, E. coli

HAV-P3C (residues 1643-1743)

PR-1121 100µg
EUR 300.7
Description: recombinant, E. coli

SARS-ACM (residues 182-216)

PR-1101 100µg
EUR 300.7
Description: recombinant, E. coli

HEV-ORF3 (residues 92-123)

PR-1187 100µg
EUR 300.7
Description: recombinant, E. coli

AF6-RBDGST (residues 1-141)

PR-398 50µg
EUR 271.4
Description: human, recombinant, E. coli

SARS-ACE (residues 1-76)

PR-1100 100µg
EUR 300.7
Description: recombinant, E. coli

HIV-1 Nef (residues 3-190)

PR-1214 100µg
EUR 300.7
Description: recombinant, E. coli

TG GRA1/p24 (residues 57-149)

PR-1244 100µg
EUR 300.7
Description: Toxoplasma gondii, recombinant, E. coli

TG GRA7/p29 (residues 24-100)

PR-1245 100µg
EUR 300.7
Description: Toxoplasma gondii, recombinant, E. coli

Clenbuterol Residues [BSA]

DAGA-004B 1mg
EUR 936

Clenbuterol Residues [HRP]

DAGA-004H 1mg
EUR 1404

Clenbuterol Residues [KLH]

DAGA-004K 1mg
EUR 1248

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

IBOCHYTLCKSG100UG each
EUR 297
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

SARS-ACN/1 (residues 340-390)

PR-1102 100µg
EUR 300.7
Description: recombinant, E. coli

HAV-VP1-P2A (residues 722-830)

PR-1113 100µg
EUR 300.7
Description: recombinant, E. coli

HAV-VP1-P2A (residues 669-782)

PR-1114 100µg
EUR 300.7
Description: recombinant, E. coli

HAV-P2C-P3B (residues 1492-1606)

PR-1118 100µg
EUR 300.7
Description: recombinant, E. coli

HAV-VP4-VP2 (residues 55-164)

PR-1120 100µg
EUR 300.7
Description: recombinant, E. coli

SARS-ACN/2 (residues 1-49)

PR-1103 100µg
EUR 300.7
Description: recombinant, E. coli

VZV ORF9 (residues 6-28/76-100)

PR-1254 100µg
EUR 300.7
Description: recombinant, E. coli

VZV ORF26 (residues 9-33/184-208)

PR-1255 100µg
EUR 300.7
Description: recombinant, E. coli

Histone H3 Peptide (residues 15-39) 

R-1005
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  • 200 µl
  • 100 ul
  • 200 ul

HBV-Core 18 kDa (residues 1-183)

PR-1123 100µg
EUR 300.7
Description: recombinant, E. coli

Histone H3 Peptide (residues 1-21) 

R-1003
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  • 400 µl
  • 200 ul
  • 400 ul

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

IBCTCLOSGPF10UG each
EUR 165
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY250UG each
EUR 784
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY50UG each
EUR 304
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

SARS-ACN/3 (residues 1-49/192-220)

PR-1104 100µg
EUR 300.7
Description: recombinant, E. coli

Clenbuterol residues ELISA Kit

DEIANJ44 96T
EUR 1039.2
Description: This ELISA kit is used for quantitative determination of Clenbuterol residues.

SARS-ACSMS(M) (residues 408-470/540-573)

PR-1106 100µg
EUR 300.7
Description: recombinant, E. coli

HTLV-1 Mosaic (residues 374-400/190-207)

PR-1221 100µg
EUR 300.7
Description: recombinant, E. coli

HBV-Core delta 14 kDa (residues 1-144)

PR-1124 100µg
EUR 300.7
Description: recombinant, E. coli

EBV-EBNA1 Mosaic (residues 1-90/408-498)

PR-1223 100µg
EUR 300.7
Description: recombinant, E. coli

RV gE1 Mosaic (residues 157-176/374-390/213-239)

PR-1228 100µg
EUR 300.7
Description: recombinant, E. coli

Histone H3 Peptide (residues 21-44), Biotinylated

R-1006
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  • 200 µl
  • 100 ul
  • 200 ul

Histone H3 Peptide (residues 69-89), Biotinylated

R-1057 200 µl
EUR 267.06

Sulfonamides residues (SAs) ELISA Kit

AE81013SF-48Tests 48 Tests
EUR 245
Description: Food Safety

Sulfonamides residues (SAs) ELISA Kit

AE81013SF-96Tests 96 Tests
EUR 450
Description: Food Safety

Sulfonamides residues (SAs) ELISA Kit

RDFS-SAs-48T 48T
EUR 488
Description: Animal tissue, aquatic tissue, honey, etc.

Sulfonamides residues (SAs) ELISA Kit

RDFS-SAs-96T 96T
EUR 698
Description: Animal tissue, aquatic tissue, honey, etc.

SARS-ACSMS(C) (residues 1051-1076/1121-1154/1162-1190)

PR-1105 100µg
EUR 300.7
Description: recombinant, E. coli

Histone H3 Peptide (residues 1-21), Biotinylated

R-1004
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  • 200 µl
  • 100 ul
  • 200 ul

Histone H4 Peptide (residues 1-21), Biotinylated

R-1007
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  • 200 µl
  • 100 ul
  • 200 ul

Sulfonamides residues ELISA Kit (OKAO00112)

OKAO00112 96 Wells
EUR 669.6
Description: Description of target: The related sulfinamides (R(S=O)NHR) are amides of sulfinic acids (R(S=O)OH) (see sulfinyl). Chiral sulfinamides such as tert-butanesulfinamide, p-toluenesulfinamide and 2,4,6-trimethylbenzenesulfinamide are relevant to asymmetric synthesis.;Species reactivity: General;Application: ;Assay info: Assay Methodology: Competitive Inhibition ELISA;Sensitivity:
ComponentAmount
Tissue0.4 ppb
Honey, egg0.4 ppb
Serum, urine1.6 ppb
Milk8 ppb

Sulfonamides residues (SAs) ELISA Test Kit

LSY-10009 96 well/kit Ask for price

Anti- HBB(Middle residues) Polyclonal Antibody

AB5FOBP2 0.1mg
EUR 399

Anti- OGCP(Middle residues) Polyclonal Antibody

AB2E152 0.1mg
EUR 399

OKAO00112-96W - Sulfonamides residues ELISA Kit

OKAO00112-96W 96Wells
EUR 475

Anti-NDR1(Middle residues) Polyclonal Antibody

AB2D122 0.1mg
EUR 399

Anti-NFKB2 (Middle residues)Polyclonal Antibody

AB2A103 0.1mg
EUR 399

Anti-IKBKB(Middle residues) Polyclonal Antibody

AB2B017 0.1mg
EUR 399

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

Anti-GDF-15(Middle residues) Polyclonal Antibody

AB1G192 0.1mg
EUR 399

Anti- Protein J5(Middle residues) Polyclonal Antibody

AB3J073 0.1mg
EUR 399

Anti-IKBKB(Near middle residues) Polyclonal Antibody

AB2B0111 0.1mg
EUR 399

Anti-IKBKB(Near middle residues) Polyclonal Antibody

AB2B019 0.1mg
EUR 399

Anti-EHV-1(Middle residues) GP2 Polyclonal Antibody

AB1G101 0.1mg
EUR 399

Anti-Capsid protein VP1(Middle residues) Polyclonal Antibody

AB2A242 0.1mg
EUR 399

Rat Tuberoinfundibular peptide of 39 residues (PTH2) ELISA Kit

abx549789-96tests 96 tests
EUR 687.5

Anti-Tail Sheath Protein(Middle residues) Polyclonal Antibody

AB2G115 0.1mg
EUR 399

Mouse Tuberoinfundibular peptide of 39 residues, Pth2 ELISA KIT

ELI-45863m 96 Tests
EUR 1038

Human Tuberoinfundibular peptide of 39 residues, PTH2 ELISA KIT

ELI-17166h 96 Tests
EUR 988.8

Human Tuberoinfundibular peptide of 39 residues (PTH2) ELISA Kit

abx549787-96tests 96 tests
EUR 687.5

Mouse Tuberoinfundibular peptide of 39 residues (PTH2) ELISA Kit

abx549788-96tests 96 tests
EUR 687.5

Bovine Tuberoinfundibular peptide of 39 residues, PTH2 ELISA KIT

ELI-28806b 96 Tests
EUR 1113.6

TruStrip RDT Sulfonamides residues (SAs) rapid test strips, results is 2-10 mins, 50 strips/pk

DE-100090-RDT 1 pk
EUR 781.2

pmTFP1-NT(N terminal tag)

ABP-FP-TNNCS 5 ug Ask for price

pLanRFP-NT(N terminal tag)

ABP-FP-RNNCS 5 ug Ask for price

pLanYFP-NT(N terminal tag)

ABP-FP-YNNCS 5 ug Ask for price

pmWasabi-NT(N terminal tag)

ABP-FP-WNNCS 5 ug Ask for price

Caspase-3 (N terminal region)

RA21012 100 ug
EUR 428.4

pmTFP1-NT(N terminal tag)-PuroR

ABP-FP-TNNCSP 5 ug Ask for price

pLanRFP-NT(N terminal tag)-PuroR

ABP-FP-RNNCSP 5 ug Ask for price

pLanYFP-NT(N terminal tag)-PuroR

ABP-FP-YNNCSP 5 ug Ask for price

pmWasabi-NT(N terminal tag)-PuroR

ABP-FP-WNNCSP 5 ug Ask for price

Cleaved GSDMD (N Terminal) Rabbit mAb

A22523 200μL
EUR 128.7
Description: A synthetic peptide corresponding to a sequence within amino acids 232-331 of human NUDC (Q9Y266).

Anti- CXCR4 N terminal Mouse Antibody

GWB-B13A74 0.2 mg Ask for price
Of the 21 sufferers, 10 had variants in LDLR, 1 in APOB with APOE, 1 in LIPC plus elevated polygenic rating, and a pair of sufferers confirmed one deletion and one duplication in LDLR, the later with a variation in LIPA. It’s highlighted that 6 of the 21 sufferers with a rating > Eight didn’t present any genetic alteration. We are able to conclude that 28% of the sufferers with particular scientific analysis of FH didn’t present genetic alteration. The doable explanations for this end result can be the presence of mutations in new genes, complicated results of the atmosphere over the genes, the gene-gene interactions, and at last the impossibility of detecting variants with the present accessible strategies.

Rapid High Throughput Whole Genome Sequencing of SARS-CoV-2 by using One-step RT-PCR Amplification with Integrated Microfluidic System and Next-Gen Sequencing

Rapid High Throughput Whole Genome Sequencing of SARS-CoV-2 by using One-step RT-PCR Amplification with Integrated Microfluidic System and Next-Gen Sequencing

The long-lasting international COVID-19 pandemic calls for well timed genomic investigation of SARS-CoV-2 viruses. Right here we report a easy and environment friendly workflow for entire genome sequencing using one-step RT-PCR amplification on a microfluidic platform, adopted by MiSeq amplicon sequencing. The strategy makes use of Fluidigm Built-in Fluidic Circuit (IFC) and devices to amplify 48 samples with 39 pairs of primers, together with 35 customized primer pairs and 4 further primer pairs from the ARTIC community protocol v3. Utility of this technique on RNA samples from each viral isolate and medical specimens show robustness and effectivity of this technique in acquiring the complete genome sequence of SARS-CoV-2.

A number of pathogens repeatedly threaten viticulture worldwide. Till now, the investigation on resistance loci has been the primary development to grasp the interplay between grapevine and the mold causal brokers. Dominantly inherited gene-based resistance has proven to be race-specific in some circumstances, to confer partial immunity, and to be doubtlessly overcome inside a couple of years since its introgression. Lately, on the footprint of analysis carried out in Arabidopsis, putative genes related to downy mildew susceptibility have been found additionally within the grapevine genome. On this work, we deep-sequenced 4 putative susceptibility genes-namely

VvDMR6.1, VvDMR6.2, VvDLO1, VvDLO2-in 190 genetically various grapevine genotypes to find new sources of broad-spectrum and recessively inherited resistance. Recognized Single Nucleotide Polymorphisms have been screened in a bottleneck evaluation from the genetic sequence to their affect on protein construction. Fifty-five genotypes confirmed not less than one impacting mutation in a number of of the scouted genes. Haplotypes have been inferred for every gene and two of them on the VvDMR6.2 gene have been discovered considerably extra represented in downy mildew resistant genotypes. The present outcomes present a useful resource for grapevine and plant genetics and will corroborate genomic-assisted breeding packages in addition to tailor-made gene enhancing approaches for resistance to biotic stresses.

Deficits within the Skeletal Muscle Transcriptome and Mitochondrial Coupling in Progressive Diabetes-Induced CKD Relate to Useful Decline

Two-thirds of these with type-2 diabetes (T2DM) have or will develop persistent kidney illness (CKD), characterised by speedy renal decline that, along with superimposed T2DM-related metabolic sequelae, synergistically promote early frailty and mobility-deficits that will increase danger of mortality. Distinguishing the mechanisms linking renal decline to mobility deficits in CKD development and/or rising severity in T2DM is instrumental in each figuring out these at high-risk for practical decline, and in formulating efficient therapy methods to stop renal failure. Moreover, muscle mitochondrial coupling is impaired as early as stage 3-CKD, with further deficits in ETC-respiration, enzymatic exercise, and elevated redox-leak.

Whereas proof means that skeletal muscle energetics could relate to the event of those comorbidities in advanced-CKD, this has by no means been assessed throughout the spectrum of CKD development, particularly in T2DM-induced CKD. Right here, utilizing subsequentgen sequencing, we first report vital downregulation in transcriptional networks governing oxidative phosphorylation, coupled electron-transport, electron-transport-chain(ETC)-complex meeting, and mitochondrial group in each middle- and late-stage CKD in T2DM. Furthermore, mitochondrial ETC operate and coupling strongly associated to muscle efficiency, and bodily operate. Our outcomes point out that T2DM-induced CKD development impairs bodily operate, with implications for altered metabolic transcriptional networks and mitochondrial practical deficits, as main mechanistic elements early in CKD-progression in T2DM.

Rapid High Throughput Whole Genome Sequencing of SARS-CoV-2 by using One-step RT-PCR Amplification with Integrated Microfluidic System and Next-Gen Sequencing

Stress induces divergent gene expression amongst lateral habenula efferent pathways

The lateral habenula (LHb) integrates vital data relating to aversive stimuli that shapes resolution making and behavioral responses. The three main LHb outputs innervate dorsal raphe nucleus (DRN), ventral tegmental space (VTA), and the rostromedial tegmental nucleus (RMTg). LHb neurons that undertaking to those targets are segregated and nonoverlapping, and this led us to think about whether or not they have distinct molecular phenotypes and variations to emphasize publicity. With a purpose to seize a time-locked profile of gene expression after repeated compelled swim stress, we used intersectional expression of RiboTag in rat LHb neurons and subsequentgen RNA sequencing to interrogate the RNAs actively present process translation from every of those pathways.

The “translatome” within the neurons comprising these pathways was related at baseline, however diverged after stress, particularly within the neurons projecting to the RMTg. Utilizing weighted gene co-expression community evaluation, we discovered one module, which had an overrepresentation of genes related to phosphoinositide Three kinase (PI3K) signaling, comprising genes downregulated after stress within the RMTg-projecting LHb neurons. Diminished PI3K signaling in RMTg-projecting LHb neurons could also be a compensatory adaptation that alters the practical stability of LHb outputs to GABAergic vs. monoaminergic neurons following repeated stress publicity.

sequencing system 20x50 cm

ESEQ1200-SYS ea
EUR 1842

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 174

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 262.8

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1988.4

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 781.2

Sequencing Grade V8 Protease

RE014 50ug
EUR 209

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 375.6

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 343.2

Sequencing Grade Modified Trypsin

RE012 100ug
EUR 229

PCR Clean Up for DNA Sequencing

BT5100 100preps
EUR 114.82

PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
EUR 553.3

Sequencing Grade Carboxypeptidase B

RE011 100ug
EUR 99

ACES Sequencing Buffer 10X, pH 7.7

21420000-1 1 L
EUR 138.62

ACES Sequencing Buffer 10X, pH 7.7

21420000-2 4 L
EUR 456.17

TBE-Buffer 5X for DNA Sequencing

40121072-1 100 mL
EUR 26.54

TBE-Buffer 5X for DNA Sequencing

40121072-2 500 mL
EUR 44.84

TBE-Buffer 5X for DNA Sequencing

40121072-3 1 L
EUR 72.29

TBE-Buffer 5X for DNA Sequencing

40121072-4 4 L
EUR 192.14

SequaGel Sequencing System 1L Kit - 1KIT

NAT1136 1KIT
EUR 187.65

SequaGel Sequencing System 2.2L Kit - EACH

NAT1138 EACH
EUR 318.6

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ100UG each
EUR 148
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ1MG each
EUR 765
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ400UG each
EUR 419
Description: Bovine Trypsin Purified Sequencing Grade

EpiNext Bisulfite Sequencing Kit (Illumina)

P-1056
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  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 576

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY100UG each
EUR 130
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY1MG each
EUR 640
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY400UG each
EUR 344
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

17068 5 nmoles
EUR 2091

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

IBOCHYTLCKSG100UG each
EUR 297
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

iLenti Sequence Primers

LV012 100 μl (10 μM)
EUR 75

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

IBCTCLOSGPF10UG each
EUR 165
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY250UG each
EUR 784
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY50UG each
EUR 304
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 10/pk
EUR 639.6
Description: Sealing Products; Sealing mats - Axygen

Human Stem Cell Primer Library

HSCL-I 1 set
EUR 540

Rat NFKappaB Primer Library

RNFKB-I 1 set
EUR 774

Rat Cytokine Primer Library I

RCA-I 1 set
EUR 657.6

Rat Cytokine Primer Library II

RCA-II 1 set
EUR 657.6

Human DNA Repair Primer Library I

HDRL-I 1 set
EUR 657.6

Human DNA Repair Primer Library II

HDRL-II 1 set
EUR 657.6

Human Cell Cycle Primer Library

HCC-I 1 set
EUR 540

Mouse Cell Cycle Primer Library

MCC-I 1 set
EUR 540

Mouse DNA Repair Primer Library I

MDRL-I 1 set
EUR 774

Mouse DNA Repair Primer Library II

MDRL-II 1 set
EUR 774

Rat Autophagy Primer Library

RATPL-I 1 set
EUR 774

Human Melanoma Primer Library

HMEL-I 1 set
EUR 657.6

Human NFKappaB Primer Library

HNFKB-I 1 set
EUR 540

Human Oncogene Primer Library

HONC-I 1 set
EUR 657.6

Human Cytokine Primer Library I

HCA-I 1 set
EUR 540

Human Cytokine Primer Library II

HCA-II 1 set
EUR 540

Mouse Cytokine Primer Library I

MCA-I 1 set
EUR 540

Mouse Cytokine Primer Library II

MCA-II 1 set
EUR 540

Mouse Oncogene Primer Library

MONC-I 1 set
EUR 657.6

Mouse NFKappaB Primer Library

MNFKB-I 1 set
EUR 540

Human Liver Cancer Primer Library

HLCPL-I 1 set
EUR 657.6

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 657.6

Human Apoptosis Primer Library

HPA-I 1 set
EUR 540

Human Autophagy Primer Library

HATPL-I 1 set
EUR 540

Human Brain Cancer Primer Library

HBRC-I 1 set
EUR 657.6

Human Colon Cancer Primer Library I

HCCP-I 1 set
EUR 657.6

Human Colon Cancer Primer Library II

HCCP-II 1 set
EUR 657.6

Mouse Apoptosis Primer Library

MPA-I 1 set
EUR 540

Mouse Autophagy Primer Library

MATPL-I 1 set
EUR 540

Rat Bdnf Signaling Primer Library

RBDNF-I 1 set
EUR 774

Rat Gdnf Signaling Primer Library

RGDNF-I 1 set
EUR 774

Human Homeobox Gene Primer Library

HHOX-I 1 set
EUR 774

Human Ras Signaling Primer Library

HRAS-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 657.6

Human TNF Signaling Primer Library

HTNF-I 1 set
EUR 657.6

Human p53 Signaling Primer Library

HTPS-I 1 set
EUR 540

Human WNT Signaling Primer Library

HWNT-I 1 set
EUR 657.6

Human BMP Signaling Primer Library

HBMP-I 1 set
EUR 657.6

Human Cell Adhesion Primer Library

HCAD-I 1 set
EUR 774

Mouse p53 Signaling Primer Library

MTPS-I 1 set
EUR 540

Mouse WNT Signaling Primer Library

MWNT-I 1 set
EUR 657.6

Mouse Ras Signaling Primer Library

MRAS-I 1 set
EUR 657.6

Mouse BMP Signaling Primer Library

MBMP-I 1 set
EUR 657.6

Human EGFR Signaling Primer Library

HEGFR-I 1 set
EUR 657.6

Human ERBB Signaling Primer Library

HERBB-I 1 set
EUR 657.6

Human Gastric Cancer Primer Library

HGCPL-I 1 set
EUR 657.6

Human GDNF Signaling Primer Library

HGDNF-I 1 set
EUR 657.6

Human IL17 Signaling Primer Library

HIL17-I 1 set
EUR 657.6

Human Necroptosis Primer Library

HNCR-I 1 set
EUR 657.6

Human Ovarian Cancer Primer Library

HOVCL-I 1 set
EUR 657.6

Human PDGF Signaling Primer Library

HPDGF-I 1 set
EUR 657.6

Human SMAD Signaling Primer Library

HSMAD-I 1 set
EUR 657.6

Human Tumor Immunity Primer Library

HTIMU-I 1 set
EUR 657.6

Human VEGF Signaling Primer Library

HVEGF-I 1 set
EUR 657.6

Human BDNF Signaling Primer Library

HBDNF-I 1 set
EUR 657.6

Human Bladder Cancer Primer Library

HBLCP-I 1 set
EUR 657.6

Human cAMP Signaling Primer Library

HCAMP-I 1 set
EUR 657.6

Human Cancer Pathway Primer Library

HCAN-I 1 set
EUR 657.6

Human CREB Signaling Primer Library

HCREB-I 1 set
EUR 657.6

Mouse Tumor Immunity Primer Library

MTIMU-I 1 set
EUR 657.6

Mouse CREB Signaling Primer Library

MCREB-I 1 set
EUR 657.6

Mouse Gdnf Signaling Primer Library

MGDNF-I 1 set
EUR 657.6

Mouse Bdnf Signaling Primer Library

MBDNF-I 1 set
EUR 657.6

Mouse EGFR Signaling Primer Library

MEGFR-I 1 set
EUR 657.6

Rat Oxidative Stress Primer Library

ROSL-I 1 set
EUR 657.6

Human Hippo Signaling Primer Pathway

HHPO-I 1 set
EUR 657.6

Human Heat Shock Protein Primer Library

HHSP-I 1 set
EUR 891.6

Human JAK/STAT Signaling Primer Library

HJAK-I 1 set
EUR 657.6

Human Osteogenesis Primer Library

HOST-I 1 set
EUR 657.6

Human Prostate Cancer Primer Library

HPRC-I 1 set
EUR 657.6

Human Cytoskeleton Primer Library

HSCK-I 1 set
EUR 774

Human TGF Beta Signaling Primer Library

HTGFB-I 1 set
EUR 657.6

Human Adipogenesis Primer Library

HADG-I 1 set
EUR 657.6

Human Angiogenesis Primer Library

HAPL-I 1 set
EUR 540

Human Cancer Driver Gene I Primer Library

HCDG-I 1 set
EUR 774

Human Cancer Driver Gene II Primer Library

HCDG-II 1 set
EUR 774

Mouse TGF Beta Signaling Primer Library

MTGFB-I 1 set
EUR 657.6

Mouse Hippo Signaling Primer Library

MHPO-I 1 set
EUR 657.6

Dietary polyphenols have proven promising results in mechanistic and preclinical research on the regulation of cardiometabolic alterations. Nonetheless, medical trials have offered contradictory outcomes, with a excessive inter-individual variability. This examine explored the position of intestine microbiota and microRNAs (miRNAs) as elements contributing to the inter-individual variability in polyphenol response. 49 topics with not less than two elements of metabolic syndrome have been divided between responders (n = 23) or non-responders (n = 26), relying on the variation charge in fasting insulin after supplementation with grape pomace (6 weeks).

The populations of chosen fecal micro organism have been estimated from fecal DNA by quantitative real-time PCR (qPCR), whereas the microbial-derived brief chain fatty acids (SCFAs) have been measured in fecal samples by fuel chromatography. MicroRNAs have been analyzed by SubsequentGen Sequencing (NGS) on a consultant pattern, adopted by focused miRNA evaluation (qPCR). Responder topics confirmed considerably decrease (p<0.05) Prevotella and Firmicutes ranges, and elevated (p<0.05) miR-222 ranges.