TIAs pathway genes and associated miRNA identification in Vinca minor: supporting aspidosperma and eburnamine alkaloids linkage via transcriptomic analysis

TIAs pathway genes and associated miRNA identification in Vinca minor: supporting aspidosperma and eburnamine alkaloids linkage via transcriptomic analysis

V. minor incorporates monomeric eburnamine-type of indole alkaloids having utilization as a neuro-medicinal plant. The biosynthetic pathway analysis using miRNAs has been the focal point for plant genomic evaluation these days and this technique is utilized to get an notion proper into a possible pathway diploma look at in V. minor as understanding of genes on this prized medicinal plant is meagrely understood. The de novo transcriptomic analysis using Illumina Subsequent gen sequencing has been carried out in glasshouse shifted plant and transformed roots to elucidate the potential non confirmed steps of terpenoid indole alkaloids (TIAs) pathway in V. minor

. A putative TIA pathway is elucidated inside the look at collectively with twelve potential TIAs biosynthetic genes. The actual miRNA associated to TIAs pathway have been acknowledged and their roles have been talked about for the first time in V. minor. The comparative analysis of transcriptomic data of glasshouse shifted plant and transformed roots confirmed that the raw reads of transformed roots have been bigger (83,740,316) as compared with glasshouse shifted plant (67,733,538). The EST-SSR prediction confirmed the utmost widespread repeats amongst glasshouse shifted plant and transformed roots, although small variation was current in trinucleotide repeats restricted to glasshouse shifted plant. The look at reveals complete 37 miRNAs which have been observed to be true and should have a process in pathway as they’re going to regulate the enlargement and alkaloid manufacturing. The identification of putative pathway genes performs an important operate in establishing linkage between Aspidosperma and Eburnamine alkaloids.

Groundwater-N air air pollution derives from agricultural and concrete actions, and compromises water top quality in shallow aquifers, inserting human and environmental properly being at risk. Nonetheless, subsurface microbiota can transform dissolved inorganic nitrogen into N2. On this look at, we surveyed the microbial group of a shallow aquifer by sampling one successfully, one piezometer and a spring inside an agricultural area that receives N-inputs of larger than 700 kg/ha per yr by way of irrigation with wastewater.

The survey was carried out all through a yr with a 16S rRNA subsequentgen technique. In parallel, we quantified the number of gene copies and transcripts related to anaerobic ammonium oxidation (anammox, hzo), nitrite-dependent anaerobic methane oxidation (n-damo, nod and pmoA) and nitrous oxide low cost (last step of denitrification, nosZ), by way of the dry and moist seasons. Our outcomes confirmed that the groundwater samples had 17.7 to 22.5 mg/L of NO3-N. The bacterial and archaeal group development was distinctive at each web site, and it remained comparatively regular over time. We verified the co-occurrence of N-transforming micro organism, which was correlated with the main target of NO2/NO3 and ORP/DO values (DO: ~3.Zero mg/L).

Near Precise-Time Identification of Newest HIV Transmissions, Transmitted Drug Resistance Mutations, and Transmission Networks by MPID-NGS in North Carolina

The identification of newest HIV-1 infections amongst of us with new diagnoses is crucial to every tailoring and assessing the impression of HIV-1 prevention strategies. We developed a Multiplexed Primer ID-Subsequent Gen Sequencing (MPID-NGS) technique to determine newest infections by measuring the intra-host viral selection over numerous areas of the HIV-1 genome, together with detecting drug resistance mutations (DRMs) and phylogenetically linked clusters. We summarize the sphere implementation of this all-in-one platform amongst people with newly acknowledged HIV-1 by the North Carolina State Laboratory of Public Effectively being in 2018.
 Complete, newest an an infection was acknowledged in 94 (35%) of 268 new diagnoses. Of us youthful than 30 years outdated and people who inject medicine have been further extra prone to be acknowledged as newest an an infection. The RT space Okay103N was most likely essentially the most usually detected DRM (roughly 15%). We found an entire of 28 clusters, and people with newest an an infection have been further extra prone to be cluster members as compared with these with continuous infections (p = 0.026). We present the quick identification of newest an an infection and pre-treatment DRMs coupled with cluster analysis that may allow prioritization of linkage to care, remedy and prevention interventions to those at highest risk of onward transmission.
TIAs pathway genes and associated miRNA identification in Vinca minor: supporting aspidosperma and eburnamine alkaloids linkage via transcriptomic analysis

Fenotipo de hipercolesterolemia acquainted definitivo con estudio genético negativo en Argentina

We developed public web sites and assets for knowledge entry, show, and evaluation of plant small RNAs. These web sites are interconnected with associated knowledge sorts. The present technology of those informatics instruments was developed for Illumina knowledge, evolving over >15 years of enhancements. Our on-line databases have custom-made net interfaces to uniquely deal with and show RNA-derived knowledge from numerous plant species, starting from Arabidopsis thaliana to wheat (Triticum sp.), together with many crop and mannequin species. The net interface shows the abundance and genomic context of knowledge for small RNAs, parallel evaluation of RNA ends (PARE)/degradome reads, RNA-seq, and even chromatin immunoprecipitation (ChIP)-seq knowledge; it additionally supplies details about doubtlessly novel transcripts (antisense transcripts, various splice isoforms, regulatory intergenic transcripts).

SequaGel MD SSCP Kit

NAT1170 1KIT
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SequaGel MD Heteroduplex Kit

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100ML SequaGel Buffer

NAT1140 100ML
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450ML SequaGel XR

NAT1160 450ML
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1L SequaGel XR

NAT1162 1L
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sequencing unit 33x45 cm

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Chymotrypsin for Sequencing grade

C4001-010 4x25ug
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Chymotrypsin for Sequencing grade

C4001-100 100ug
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Trypin for Mass & Sequencing

T9600-025 25ug
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Trypin for Mass & Sequencing

T9600-100 100ug
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Trypin for Mass & Sequencing

T9600-112 12x100ug
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Trypin for Mass & Sequencing

T9600-400 4x100ug
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450ML SequaGel UreaGel Concentrate

NAT1132 450ML
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450ML SequaGel UreaGel Diluent

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1L SequaGel UreaGel Diluent

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90ML SequaGel Complete Buffer

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100ML SequaGel XR Concentrate

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PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
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PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 2798

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 480

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
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PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT 1 Kit
EUR 4335

200ML SequaGel UreaGel Complete Buffer

NAT1158 200ML
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200ML SequaGel MD Monomer Solution

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PCR Clean Up for DNA Sequencing

BT5100 100preps
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PCR Clean Up for DNA Sequencing

BT5101 1000Preps, 1000prep
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1.2ML SequaGel MD SSCP Stop Solution

NAT1174 1.2ML
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Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
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ProtoBlock System

NAT1004 1KIT
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CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

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Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

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T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

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Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
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Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

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Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

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Genorise® Customized PCR primers for cloning and sequencing

GR108023 2 nmol
EUR 65

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132

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CAS770A-KIT 10 rxn
EUR 1132

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EUR 1132

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132

data acquisition system

D230 ea
EUR 1704

photo documentation system

VLBPCX4-20M ea
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photo documentation system

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Fast Mutagenesis System

20-abx098073
  • EUR 509.00
  • EUR 592.00
  • 10 rxns
  • 20 rxns

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3600100 1unit
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4250-050-ES 1 Kit
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4252-040-02 1 Each
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CT356 10-plate
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AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 428

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 394

UreaGel 29:1 System 1L Kit

NAT1128 EACH
EUR 143

SBA Clonotyping System-AP Kit (OKSB00002)

OKSB00002 1 Kit
EUR 846
Description: Description of target: ;Species reactivity: ;Application: ELISA, ELISPOT;Assay info: ;Sensitivity:

SBA Clonotyping System Kit : HRP (OKSB00003)

OKSB00003 1 Kit
EUR 787
Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity:

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT 1 kit
EUR 2152
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT 10 rxn
EUR 445

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1152

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SD5011 1 UNIT
EUR 324.05

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EVS1100-MULTI ea
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modular system 20x10 cm

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amfiRivert Reverse Transcription System

R5200-050 50 rxns
EUR 388

myGel Mini Electrophoresis System

BM0286 System
EUR 618

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abx098861-10rxns 10 rxns
EUR 690

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 176

cfMAX cfDNA Isolation System

CFMAX100A-1 1 Kit
EUR 479

FractionPREP Cell Fractionation system

55R-1359 50 assays
EUR 758
Description: Cell Fractionation system for use in the research laboratory

BCIP/NBT Substrate System

GR103022 2 x 25 mL
EUR 119

AP Fluorescent Substrate System

GR103053 Each
EUR 83

HRP Floresce Substrate System

GR103054 Each
EUR 83

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SC900A-1 5 reactions
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EpiNext DNA Purification HT System

P-1063 480 ml
EUR 6022.55
Description: The best epigenetics products

mini vertical system 10x10 cm

EVS1100-SYS ea
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AXYGEN® GEL DOCUMENTATION SYSTEM

GD-1000 1/pk
EUR 4670
Description: Lab Equipment; Axygen Branded EQ

data acquisition system 120 vac

D230-US ea
EUR 1704

ViraSafe Lentiviral Packaging System, Ecotropic

VPK-205 1 kit
EUR 891
Description: Our ViraSafe Lentiviral Packaging Systems contain three packaging plasmids for co-transfection with your own third generation lentiviral expression construct. If you need an expression vector as well as the packaging plasmids, consider our ViraSafe Lentiviral Expression Complete Systems. Our Ecotropic Packaging System assembles lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedure

ViraSafe Lentiviral Packaging System, Pantropic

VPK-206 1 kit
EUR 891
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Platinum Retroviral Expression System, Ecotropic

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EUR 1169
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-GP Packaging cell line which contains the gag and pol genes necessary for viral packaging. Simply clone your gene of interest into the vector provided and co-transfect into the packaging cells along with the included VSVG plasmid.

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  • EUR 6759.00
  • EUR 5924.00
  • EUR 4281.00
  • 100 ml
  • 500 ml
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HISTAR DETECTION SYSTEM Kits (OKSA11259)

OKSA11259 50 Tests
EUR 565
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11260)

OKSA11260 150 Tests
EUR 1335
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

HISTAR DETECTION SYSTEM Kits (OKSA11261)

OKSA11261 500 Tests
EUR 2019
Description: Description of target: HISTAR DETECTION SYSTEM;Species reactivity: ;Application: IHC-AFF, IHC-FFPE;Assay info: ;Sensitivity:

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 356

Slidefile Storage System 100 Positions

M700-100W-WHITE 1 Case(s)
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Stain Tray Slide Staining System

M920-1 1 Clear
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Stain Tray Slide Staining System

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Gryphon Retroviral Expression System I

ABP-RVK-10001 1 set Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

Gryphon Retroviral Expression System II

ABP-RVK-10002 1 set Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody

EXOAB-KIT-1 25 ul each
EUR 627

Human Visual system homeobox 1 (VSX1) ELISA Kit

abx384263-96tests 96 tests
EUR 911

Human Visual system homeobox 2 (VSX2) ELISA Kit

abx384264-96tests 96 tests
EUR 911

Super Sensitive IHC Detection System Kit (Mouse / Rabbit)

abx097195-1Kit 1 Kit
EUR 356

SBA Clonotyping System-B6/C57J-AP Kit (OKSB00005)

OKSB00005 1 Kit
EUR 852
Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity:

SBA Clonotyping System-B6/C57J Kit : HRP (OKSB00006)

OKSB00006 1 Kit
EUR 800
Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity:

Mouse Visual system homeobox 2, Vsx2 ELISA KIT

ELI-51409m 96 Tests
EUR 865

Mouse Visual system homeobox 1, Vsx1 ELISA KIT

ELI-51425m 96 Tests
EUR 865

Human Visual system homeobox 1, VSX1 ELISA KIT

ELI-44479h 96 Tests
EUR 824

Human Visual system homeobox 2, VSX2 ELISA KIT

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Chicken Visual system homeobox 2, VSX2 ELISA KIT

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EUR 928

Chicken Visual system homeobox 1, VSX1 ELISA KIT

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Bovine Visual system homeobox 1, VSX1 ELISA KIT

ELI-40589b 96 Tests
EUR 928

Dog Visual system homeobox 1(VSX1) ELISA kit

E08V0077-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Visual system homeobox 1(VSX1) ELISA kit

E08V0077-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Visual system homeobox 1(VSX1) ELISA kit

E08V0077-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Visual system homeobox 2(VSX2) ELISA kit

E08V0078-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Visual system homeobox 2(VSX2) ELISA kit

E08V0078-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Visual system homeobox 2(VSX2) ELISA kit

E08V0078-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Visual system homeobox 1(VSX1) ELISA kit

E09V0077-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Visual system homeobox 1(VSX1) ELISA kit

E09V0077-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Visual system homeobox 1(VSX1) ELISA kit

E09V0077-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Visual system homeobox 2(VSX2) ELISA kit

E09V0078-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Quite a few choices are included for downloading knowledge as tables or through net providers. Interpretation of those knowledge is facilitated by the inclusion of intensive repeat or transposon knowledge in our genome viewer. We now have developed graphical and analytical instruments, together with a brand new viewer and a question web page for the evaluation of phased small RNAs; these are significantly helpful for understanding the complicated small RNA pathways of vegetation