Serological screening of sexual assault proof has historically targeted on enzyme exercise and immunochromatographic assays that present solely a presumptive indication of seminal fluid and have restricted sensitivity relative to DNA testing. Seminal fluid detection primarily based on protein mass spectrometry represents a “Subsequent Gen” serological expertise that overcomes the specificity and sensitivity limitations of conventional serological screening however requires time-consuming pattern preparation protocols.
This paper describes a novel “peptidomics” strategy to seminal fluid detection that eliminates the necessity for prolonged trypsin digestion. This streamlines pattern preparation to a one-step course of adopted by high-resolution mass spectrometry to establish naturally occurring seminal fluid peptides and low-molecular weight proteins. A number of protein biomarkers of seminal fluid had been persistently and confidently recognized primarily based on the multiplexed detection of quite a few endogenous peptides. This helps the forensic applicability of a peptidomic assay for seminal fluid identification with same-day pattern preparation and evaluation. Our knowledge counsel the predominance of solute service genes throughout metabolic reprogramming of prostate most cancers cells in an androgen-deprived atmosphere, thus signifying them as doubtlessly engaging therapeutic targets.
These included Semenogelin I and II (90% and 86% sequence protection, respectively); Prostate Particular Antigen/p30 (29% sequence protection); and Prostatic Acid Phosphatase (24% sequence protection). The efficiency of this streamlined peptidomics strategy to seminal fluid identification in a forensic context was additionally assessed utilizing simulated casework samples of the kind sometimes collected as a part of a sexual assault examination (e.g., oral and vaginal swabs stained with semen). The ensuing knowledge reveal that sub-microliter portions of seminal fluid on cotton swabs may be recovered and reliably detected. Future improvement and streamlined multiplex peptidomic assays for added organic stains can simply be envisaged.
Metabolic Reprogramming and Predominance of Solute Provider Genes throughout Acquired Enzalutamide Resistance in Prostate Most cancers
Androgen deprivation remedy (ADT) is standard-of-care for advanced-stage prostate most cancers, and enzalutamide (Xtandi®, Astellas, Northbrook, IL, USA), a second technology antiandrogen, is prescribed on this scientific setting. The response to this remedy is normally short-term with the speedy emergence of drug resistance. A greater understanding of gene expression adjustments related to enzalutamide resistance will facilitate circumventing this downside. We in contrast the transcriptomic profile of paired enzalutamide-sensitive and resistant LNCaP and C4-2B prostate most cancers cells for identification of genes concerned in drug resistance by performing an unbiased bioinformatics evaluation and additional validation.
Subsequent–Gensequencing detected 9409 and 7757 genes differentially expressed in LNCaP and C4-2B cells, in comparison with their parental counterparts. A subset of differentially expressed genes had been validated by qRT-PCR. Evaluation by the i-pathway revealed membrane transporters together with solute service proteins, ATP-binding cassette transporters, and drug metabolizing enzymes as essentially the most distinguished genes dysregulated in resistant cell traces. RNA-Seq knowledge demonstrated predominance of solute service genes SLC12A5, SLC25A17, and SLC27A6 throughout metabolic reprogramming and improvement of drug resistance. Upregulation of those genes had been related to increased uptake of lactic/citric acid and decrease glucose consumption in resistant cells.
Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells
The channel catfish (Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the usual cell line used for channel catfish diagnostics. Subsequent–gen sequencing research of a virus cultured within the CCO cells revealed mitochondrial sequences matching these of brown bullhead (Ameiurus nebulosus, Lesueur). Due to this fact, we systematically carried out partial cytochrome oxidase 1 gene sequencing of a number of sources of the CCO cell line and all matched the brown bullhead and never the channel cat. After evaluating the chosen substrates for Prevotella and goal genes of miR-222, these variations urged that responders had been these topics who exhibited impaired glycaemic management. This examine reveals that fecal microbiota and miRNA expression could also be associated to inter-individual variability in scientific trials with polyphenols. This text is protected by copyright. All rights reserved.
Phenotype of particular familial hypercholesterolemia with damaging genetic examine in Argentina
sequencing system 20x50 cm |
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ESEQ1200-SYS | Consort | ea | EUR 1842 |
Trypin for Mass & Sequencing |
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T9600-025 | GenDepot | 25ug | EUR 174 |
Trypin for Mass & Sequencing |
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T9600-100 | GenDepot | 100ug | EUR 262.8 |
Trypin for Mass & Sequencing |
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T9600-112 | GenDepot | 12x100ug | EUR 1988.4 |
Trypin for Mass & Sequencing |
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T9600-400 | GenDepot | 4x100ug | EUR 781.2 |
Sequencing Grade V8 Protease |
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RE014 | SAB | 50ug | EUR 209 |
Chymotrypsin for Sequencing grade |
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C4001-010 | GenDepot | 4x25ug | EUR 375.6 |
Chymotrypsin for Sequencing grade |
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C4001-100 | GenDepot | 100ug | EUR 343.2 |
Sequencing Grade Modified Trypsin |
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RE012 | SAB | 100ug | EUR 229 |
PCR Clean Up for DNA Sequencing |
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BT5100 | Bio Basic | 100preps | EUR 114.82 |
PCR Clean Up for DNA Sequencing |
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BT5101 | Bio Basic | 1000Preps, 1000prep | EUR 553.3 |
Sequencing Grade Carboxypeptidase B |
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RE011 | SAB | 100ug | EUR 99 |
ACES Sequencing Buffer 10X, pH 7.7 |
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21420000-1 | Glycomatrix | 1 L | EUR 138.62 |
ACES Sequencing Buffer 10X, pH 7.7 |
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21420000-2 | Glycomatrix | 4 L | EUR 456.17 |
HCV-N-G3/10 (residues 2-119) |
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PR-1140 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
TBE-Buffer 5X for DNA Sequencing |
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40121072-1 | Glycomatrix | 100 mL | EUR 26.54 |
TBE-Buffer 5X for DNA Sequencing |
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40121072-2 | Glycomatrix | 500 mL | EUR 44.84 |
TBE-Buffer 5X for DNA Sequencing |
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40121072-3 | Glycomatrix | 1 L | EUR 72.29 |
TBE-Buffer 5X for DNA Sequencing |
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40121072-4 | Glycomatrix | 4 L | EUR 192.14 |
SequaGel Sequencing System 1L Kit - 1KIT |
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NAT1136 | Scientific Laboratory Supplies | 1KIT | EUR 187.65 |
SequaGel Sequencing System 2.2L Kit - EACH |
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NAT1138 | Scientific Laboratory Supplies | EACH | EUR 318.6 |
Bovine Trypsin Purified Sequencing Grade |
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IBOTRYSEQ100UG | Innovative research | each | EUR 148 |
Description: Bovine Trypsin Purified Sequencing Grade |
Bovine Trypsin Purified Sequencing Grade |
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IBOTRYSEQ1MG | Innovative research | each | EUR 765 |
Description: Bovine Trypsin Purified Sequencing Grade |
Bovine Trypsin Purified Sequencing Grade |
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IBOTRYSEQ400UG | Innovative research | each | EUR 419 |
Description: Bovine Trypsin Purified Sequencing Grade |
EpiNext Bisulfite Sequencing Kit (Illumina) |
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P-1056 | EpiGentek |
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DNA Library Prep Kit for IIlumina Sequencing |
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K1475-12 | Biovision | 12 Rxns | EUR 576 |
HCV-N-G4 (residues 2-119) |
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PR-1137 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HCV-N-G5 (residues 2-119) |
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PR-1138 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HCV-N-G1a (residues 2-119) |
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PR-1130 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HCV-N-G1b (residues 2-119) |
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PR-1132 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HCV-N-G2b (residues 2-119) |
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PR-1134 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HCV-N-G3a (residues 2-119) |
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PR-1135 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HCV-N-G3b (residues 2-119) |
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PR-1136 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HCV-N-G6a (residues 2-119) |
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PR-1139 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
Bovine Trypsin Purified Sequencing Grade Lyophilized |
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IBOTRYSEQLY100UG | Innovative research | each | EUR 130 |
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized |
Bovine Trypsin Purified Sequencing Grade Lyophilized |
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IBOTRYSEQLY1MG | Innovative research | each | EUR 640 |
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized |
Bovine Trypsin Purified Sequencing Grade Lyophilized |
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IBOTRYSEQLY400UG | Innovative research | each | EUR 344 |
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized |
HCV-N-G2a (residues 2-119)GST |
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PR-1133 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
Genorise® Customized PCR primers for cloning and sequencing |
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GR108023 | Genorise Scientific | 2 nmol | EUR 65 |
RV gE2 (residues 31-105) |
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PR-1229 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
RV-C (residues 1-123) |
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PR-1230 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
TG MIC3 (residues 234-306) |
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PR-1243 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: Toxoplasma gondii, recombinant, E. coli |
Anti-TAF2(N-terminal-middle residues) Polyclonal Antibody |
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AB1J129 | Antagene | 0.1mg | EUR 399 |
EBV-EA (residues 306-390) |
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PR-1224 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
CMV pp28 (residues 130-160) |
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PR-1248 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
CMV pp65 (residues 297-510) |
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PR-1251 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HAV-P2C (residues 1121-1234) |
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PR-1115 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HAV-P3C (residues 1643-1743) |
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PR-1121 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
SARS-ACM (residues 182-216) |
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PR-1101 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HEV-ORF3 (residues 92-123) |
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PR-1187 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
AF6-RBDGST (residues 1-141) |
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PR-398 | Jena Bioscience GmbH | 50µg | EUR 271.4 |
Description: human, recombinant, E. coli |
SARS-ACE (residues 1-76) |
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PR-1100 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HIV-1 Nef (residues 3-190) |
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PR-1214 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
TG GRA1/p24 (residues 57-149) |
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PR-1244 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: Toxoplasma gondii, recombinant, E. coli |
TG GRA7/p29 (residues 24-100) |
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PR-1245 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: Toxoplasma gondii, recombinant, E. coli |
Clenbuterol Residues [BSA] |
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DAGA-004B | Creative Diagnostics | 1mg | EUR 936 |
Clenbuterol Residues [HRP] |
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DAGA-004H | Creative Diagnostics | 1mg | EUR 1404 |
Clenbuterol Residues [KLH] |
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DAGA-004K | Creative Diagnostics | 1mg | EUR 1248 |
Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade |
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IBOCHYTLCKSG100UG | Innovative research | each | EUR 297 |
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade |
SARS-ACN/1 (residues 340-390) |
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PR-1102 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HAV-VP1-P2A (residues 722-830) |
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PR-1113 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HAV-VP1-P2A (residues 669-782) |
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PR-1114 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HAV-P2C-P3B (residues 1492-1606) |
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PR-1118 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HAV-VP4-VP2 (residues 55-164) |
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PR-1120 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
SARS-ACN/2 (residues 1-49) |
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PR-1103 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
VZV ORF9 (residues 6-28/76-100) |
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PR-1254 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
VZV ORF26 (residues 9-33/184-208) |
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PR-1255 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
Histone H3 Peptide (residues 15-39) |
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R-1005 | EpiGentek |
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HBV-Core 18 kDa (residues 1-183) |
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PR-1123 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
Histone H3 Peptide (residues 1-21) |
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R-1003 | EpiGentek |
|
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Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified |
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IBCTCLOSGPF10UG | Innovative research | each | EUR 165 |
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified |
Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
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IBCTV8SEQLY250UG | Innovative research | each | EUR 784 |
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
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IBCTV8SEQLY50UG | Innovative research | each | EUR 304 |
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
SARS-ACN/3 (residues 1-49/192-220) |
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PR-1104 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
Clenbuterol residues ELISA Kit |
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DEIANJ44 | Creative Diagnostics | 96T | EUR 1039.2 |
Description: This ELISA kit is used for quantitative determination of Clenbuterol residues. |
SARS-ACSMS(M) (residues 408-470/540-573) |
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PR-1106 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HTLV-1 Mosaic (residues 374-400/190-207) |
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PR-1221 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
HBV-Core delta 14 kDa (residues 1-144) |
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PR-1124 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
EBV-EBNA1 Mosaic (residues 1-90/408-498) |
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PR-1223 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
RV gE1 Mosaic (residues 157-176/374-390/213-239) |
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PR-1228 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
Histone H3 Peptide (residues 21-44), Biotinylated |
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R-1006 | EpiGentek |
|
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Histone H3 Peptide (residues 69-89), Biotinylated |
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R-1057 | EpiGentek | 200 µl | EUR 267.06 |
Sulfonamides residues (SAs) ELISA Kit |
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AE81013SF-48Tests | Abebio | 48 Tests | EUR 245 |
Description: Food Safety |
Sulfonamides residues (SAs) ELISA Kit |
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AE81013SF-96Tests | Abebio | 96 Tests | EUR 450 |
Description: Food Safety |
Sulfonamides residues (SAs) ELISA Kit |
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RDFS-SAs-48T | Reddot Biotech | 48T | EUR 488 |
Description: Animal tissue, aquatic tissue, honey, etc. |
Sulfonamides residues (SAs) ELISA Kit |
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RDFS-SAs-96T | Reddot Biotech | 96T | EUR 698 |
Description: Animal tissue, aquatic tissue, honey, etc. |
SARS-ACSMS(C) (residues 1051-1076/1121-1154/1162-1190) |
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PR-1105 | Jena Bioscience GmbH | 100µg | EUR 300.7 |
Description: recombinant, E. coli |
Histone H3 Peptide (residues 1-21), Biotinylated |
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R-1004 | EpiGentek |
|
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Histone H4 Peptide (residues 1-21), Biotinylated |
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R-1007 | EpiGentek |
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Sulfonamides residues ELISA Kit (OKAO00112) |
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OKAO00112 | Aviva Systems Biology | 96 Wells | EUR 669.6 | ||||||||||
Description: Description of target: The related sulfinamides (R(S=O)NHR) are amides of sulfinic acids (R(S=O)OH) (see sulfinyl). Chiral sulfinamides such as tert-butanesulfinamide, p-toluenesulfinamide and 2,4,6-trimethylbenzenesulfinamide are relevant to asymmetric synthesis.;Species reactivity: General;Application: ;Assay info: Assay Methodology: Competitive Inhibition ELISA;Sensitivity:
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Sulfonamides residues (SAs) ELISA Test Kit |
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LSY-10009 | Shenzhen Lvshiyuan Biotechnology | 96 well/kit | Ask for price |
Anti- HBB(Middle residues) Polyclonal Antibody |
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AB5FOBP2 | Antagene | 0.1mg | EUR 399 |
Anti- OGCP(Middle residues) Polyclonal Antibody |
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AB2E152 | Antagene | 0.1mg | EUR 399 |
OKAO00112-96W - Sulfonamides residues ELISA Kit |
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OKAO00112-96W | Aviva Systems Biology | 96Wells | EUR 475 |
Anti-NDR1(Middle residues) Polyclonal Antibody |
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AB2D122 | Antagene | 0.1mg | EUR 399 |
Anti-NFKB2 (Middle residues)Polyclonal Antibody |
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AB2A103 | Antagene | 0.1mg | EUR 399 |
Anti-IKBKB(Middle residues) Polyclonal Antibody |
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AB2B017 | Antagene | 0.1mg | EUR 399 |
SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK |
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AM-96-SEPTA-3100 | CORNING | 10/pk | EUR 639.6 |
Description: Sealing Products; Sealing mats - Axygen |
Anti-GDF-15(Middle residues) Polyclonal Antibody |
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AB1G192 | Antagene | 0.1mg | EUR 399 |
Anti- Protein J5(Middle residues) Polyclonal Antibody |
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AB3J073 | Antagene | 0.1mg | EUR 399 |
Anti-IKBKB(Near middle residues) Polyclonal Antibody |
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AB2B0111 | Antagene | 0.1mg | EUR 399 |
Anti-IKBKB(Near middle residues) Polyclonal Antibody |
|||
AB2B019 | Antagene | 0.1mg | EUR 399 |
Anti-EHV-1(Middle residues) GP2 Polyclonal Antibody |
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AB1G101 | Antagene | 0.1mg | EUR 399 |
Anti-Capsid protein VP1(Middle residues) Polyclonal Antibody |
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AB2A242 | Antagene | 0.1mg | EUR 399 |
Rat Tuberoinfundibular peptide of 39 residues (PTH2) ELISA Kit |
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abx549789-96tests | Abbexa | 96 tests | EUR 687.5 |
Anti-Tail Sheath Protein(Middle residues) Polyclonal Antibody |
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AB2G115 | Antagene | 0.1mg | EUR 399 |
Mouse Tuberoinfundibular peptide of 39 residues, Pth2 ELISA KIT |
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ELI-45863m | Lifescience Market | 96 Tests | EUR 1038 |
Human Tuberoinfundibular peptide of 39 residues, PTH2 ELISA KIT |
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ELI-17166h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Tuberoinfundibular peptide of 39 residues (PTH2) ELISA Kit |
|||
abx549787-96tests | Abbexa | 96 tests | EUR 687.5 |
Mouse Tuberoinfundibular peptide of 39 residues (PTH2) ELISA Kit |
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abx549788-96tests | Abbexa | 96 tests | EUR 687.5 |
Bovine Tuberoinfundibular peptide of 39 residues, PTH2 ELISA KIT |
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ELI-28806b | Lifescience Market | 96 Tests | EUR 1113.6 |
TruStrip RDT Sulfonamides residues (SAs) rapid test strips, results is 2-10 mins, 50 strips/pk |
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DE-100090-RDT | Alpha Diagnostics | 1 pk | EUR 781.2 |
pmTFP1-NT(N terminal tag) |
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ABP-FP-TNNCS | Allele Biotech | 5 ug | Ask for price |
pLanRFP-NT(N terminal tag) |
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ABP-FP-RNNCS | Allele Biotech | 5 ug | Ask for price |
pLanYFP-NT(N terminal tag) |
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ABP-FP-YNNCS | Allele Biotech | 5 ug | Ask for price |
pmWasabi-NT(N terminal tag) |
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ABP-FP-WNNCS | Allele Biotech | 5 ug | Ask for price |
Caspase-3 (N terminal region) |
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RA21012 | Neuromics | 100 ug | EUR 428.4 |
pmTFP1-NT(N terminal tag)-PuroR |
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ABP-FP-TNNCSP | Allele Biotech | 5 ug | Ask for price |
pLanRFP-NT(N terminal tag)-PuroR |
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ABP-FP-RNNCSP | Allele Biotech | 5 ug | Ask for price |
pLanYFP-NT(N terminal tag)-PuroR |
|||
ABP-FP-YNNCSP | Allele Biotech | 5 ug | Ask for price |
pmWasabi-NT(N terminal tag)-PuroR |
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ABP-FP-WNNCSP | Allele Biotech | 5 ug | Ask for price |
Cleaved GSDMD (N Terminal) Rabbit mAb |
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A22523 | Abclonal | 200μL | EUR 128.7 |
Description: A synthetic peptide corresponding to a sequence within amino acids 232-331 of human NUDC (Q9Y266). |
Anti- CXCR4 N terminal Mouse Antibody |
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GWB-B13A74 | GenWay Biotech | 0.2 mg | Ask for price |